Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2 nanocomposites to suppress reactive oxygen species

被引：30

作者：

Deng, Guoying ^{[1
]}

Niu, Kerun ^{[2
]}

Zhou, Feng ^{[2
]}

Li, Buxiao ^{[1
]}

Kang, Yingjie ^{[3
]}

Liu, Xijian ^{[4
]}

Hu, Junqing ^{[5
]}

Li, Bo ^{[6
]}

Wang, Qiugen ^{[1
]}

Yi, Chengqing ^{[7
]}

Wang, Qian ^{[1
,5
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Sch Med, Trauma Ctr, 650 Xin Songjiang Rd, Shanghai 201620, Peoples R China

[2] Nanjing Med Univ, Shanghai Gen Hosp, Shanghai Bone Tumor Inst, Dept Orthoped, Shanghai 200080, Peoples R China

[3] Shanghai Univ Tradit Chinese Medicine, Shuguang Hosp, Dept Radiol, 528 Zhangheng Rd, Shanghai 201203, Peoples R China

[4] Shanghai Univ Engn Sci, Coll Chem & Chem Engn, Shanghai 201620, Peoples R China

[5] Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China

[6] Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, 1295 Dingxi Rd, Shanghai 200050, Peoples R China

[7] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Sch Med, Shanghai Bone Tumor Inst,Dept Orthoped, 100 Haining Rd, Shanghai 200080, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

OXIDATIVE-STRESS; SELENIUM NANOPARTICLES; ELEMENTAL SELENIUM; AVASCULAR NECROSIS; NONTRAUMATIC OSTEONECROSIS; NANO-SE; TOXICITY; DAMAGE; SIZE; ACTIVATION;

D O I：

10.1038/srep43914

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Reducing oxidative stress (ROS) have been demonstrated effective for steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH). Selenium (Se) plays an important role in suppressing oxidative stress and has huge potential in ONFH treatments. However the Se has a narrow margin between beneficial and toxic effects which make it hard for therapy use in vivo. In order to make the deficiency up, a control release of Se (Se@SiO2) were realized by nanotechnology modification. Porous Se@SiO2 nanocomposites have favorable biocompatibility and can reduced the ROS damage effectively. In vitro, the cck-8 analysis, terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) stain and flow cytometry analysis showed rare negative influence by porous Se@SiO2 nanocomposites but significantly protective effect against H2O2 by reducing ROS level (detected by DCFH-DA). In vivo, the biosafety of porous Se@SiO2 nanocomposites were confirmed by the serum biochemistry, the ROS level in serum were significantly reduced and the curative effect were confirmed by Micro CT scan, serum Elisa assay inflammatory factors), Western blotting (quantitative measurement of ONFH) and HE staining. It is expected that the porous Se@SiO2 nanocomposites may prevent steroid-induced ONFH by reducing oxidative stress.

引用

页数：13

共 63 条

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Treatment with acetylsalicylic acid prevents short to mid-term radiographic progression of nontraumatic osteonecrosis of the femoral head: a pilot study [J].