HLA-B27 and Human β2-Microglobulin Affect the Gut Microbiota of Transgenic Rats

被引：183

作者：

Lin, Phoebe ^{[1
]}

Bach, Mary ^{[3
]}

Asquith, Mark ^{[2
]}

Lee, Aaron Y. ^{[4
]}

Akileswaran, Lakshmi ^{[5
]}

Stauffer, Patrick ^{[1
]}

Davin, Sean ^{[1
]}

Pan, Yuzhen ^{[1
]}

Cambronne, Eric D. ^{[9
]}

Dorris, Martha ^{[6
]}

Debelius, Justine W. ^{[7
]}

Lauber, Christian L. ^{[7
]}

Ackermann, Gail ^{[7
]}

Baeza, Yoshiki V. ^{[7
]}

Gill, Tejpal ^{[10
]}

Knight, Rob ^{[7
,11
]}

Colbert, Robert A. ^{[10
]}

Taurog, Joel D. ^{[6
]}

Van Gelder, Russell N. ^{[5
]}

Rosenbaum, James T. ^{[1
,2
,8
]}

机构：

[1] Oregon Hlth & Sci Univ, Casey Eye Inst, Portland, OR 97201 USA

[2] Oregon Hlth & Sci Univ, Div Rheumatol, Portland, OR 97201 USA

[3] Univ Washington, Div Rheumatol, VA Med Ctr, Seattle, WA 98195 USA

[4] Moorfields Eye Inst London, London, England

[5] Univ Washington, Dept Ophthalmol, Seattle, WA 98195 USA

[6] Univ Texas Southwestern, Dept Rheumatol, Dallas, TX USA

[7] Univ Colorado, Boulder, CO 80309 USA

[8] Devers Eye Inst, Portland, OR USA

[9] Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97201 USA

[10] NIAMSD, Pediat Translat Res Branch, NIH, Baltimore, MD USA

[11] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA

来源：

PLOS ONE | 2014年 / 9卷 / 08期

关键词：

HUMAN MONOCYTIC CELLS; KLEBSIELLA-PNEUMONIAE NITROGENASE; ANKYLOSING-SPONDYLITIS; HLA-B27-TRANSGENIC RATS; SALMONELLA-ENTERITIDIS; BACTEROIDES-VULGATUS; INFLAMMATORY DISEASE; AUTOIMMUNE-DISEASE; REACTIVE ARTHRITIS; MOLECULAR MIMICRY;

D O I：

10.1371/journal.pone.0105684

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The HLA-B27 gene is a major risk factor for clinical diseases including ankylosing spondylitis, acute anterior uveitis, reactive arthritis, and psoriatic arthritis, but its mechanism of risk enhancement is not completely understood. The gut microbiome has recently been shown to influence several HLA-linked diseases. However, the role of HLA-B27 in shaping the gut microbiome has not been previously investigated. In this study, we characterize the differences in the gut microbiota mediated by the presence of the HLA-B27 gene. We identified differences in the cecal microbiota of Lewis rats transgenic for HLA-B27 and human beta 2-microglobulin (h beta 2m), compared with wild-type Lewis rats, using biome representational in situ karyotyping (BRISK) and 16S rRNA gene sequencing. 16S sequencing revealed significant differences between transgenic animals and wild type animals by principal coordinates analysis. Further analysis of the data set revealed an increase in Prevotella spp. and a decrease in Rikenellaceae relative abundance in the transgenic animals compared to the wild type animals. By BRISK analysis, species-specific differences included an increase in Bacteroides vulgatus abundance in HLA-B27/h beta 2m and h beta 2m compared to wild type rats. The finding that HLA-B27 is associated with altered cecal microbiota has not been shown before and can potentially provide a better understanding of the clinical diseases associated with this gene.

引用

页数：9

共 55 条

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