Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes

被引:25
作者
Goardon, Nicolas [1 ,2 ]
Nikolousis, Emmanouil [3 ,4 ]
Sternberg, Alexander [1 ,2 ,3 ,4 ,5 ]
Chu, Wai-Kit [6 ]
Craddock, Charles [3 ,4 ]
Richardson, Peter [4 ,9 ]
Benson, Richard [7 ]
Drayson, Mark [4 ,9 ]
Standen, Graham [8 ]
Vyas, Paresh [1 ,2 ]
Freeman, Sylvie [3 ,4 ,9 ]
机构
[1] John Radcliffe Hosp, Weatherall Inst Mol Med, MRC Mol Haematol Unit, Oxford, England
[2] John Radcliffe Hosp, Weatherall Inst Mol Med, Dept Haematol, Oxford, England
[3] Univ Birmingham, Dept Haematol, Birmingham B15 2TT, W Midlands, England
[4] Univ Hosp Birmingham, Birmingham, W Midlands, England
[5] Great Western Hosp, Dept Haematol, Swindon, Wilts, England
[6] John Radcliffe Hosp, Weatherall Inst Mol Med, Canc Res UK, Oxford, England
[7] Nuffield Orthopaed Hosp, Nuffield Dept Orthopaed, Oxford, England
[8] Bristol Royal Infirm & Gen Hosp, Dept Haematol, Bristol, Avon, England
[9] Univ Birmingham, Dept Immunol, Birmingham B15 2TT, W Midlands, England
来源
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL | 2009年 / 94卷 / 08期
基金
英国医学研究理事会;
关键词
myelodyplasia; immunophenotype; FACS diagnosis; FLOW-CYTOMETRY; ABNORMALITIES; PROGENITORS; IMMATURE; UTILITY;
D O I
10.3324/haematol.2008.004085
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Diagnosis of myelodysplastic syndrome can be difficult especially in cases with a low blast count and a normal karyotype. Flow cytometry has been used to distinguish myelodysplastic syndrome from non-clonal cytopenias. No one single simple flow cytometric parameter has been proposed to be diagnostic of myelodysplastic syndrome. We have studied samples from 100 myelodysplastic syndrome patients and as control samples; 70 non-clonal cytopenias, 5 subjects with normal hematology, 31 patients with acute myeloid leukemia and 11 with chronic myelomonocytic leukemia or myeloproliferative disorder. We show that reduced relative mean fluorescence of CD38 below a threshold value on CD34(+) cells diagnosed low-grade myelodysplastic syndrome with 95% sensitivity (95% confidence interval, 87-99%) and 92% specificity (95% confidence interval, 82-97%). This simple flow cytometric test may be of value in the routine clinical diagnosis of myelodysplastic syndrome, especially in cases with a low blast count and normal karyotype.
引用
收藏
页码:1160 / 1163
页数:4
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