Jagged1 is essential for osteoblast development during maxillary ossification

被引：40

作者：

Hill, Cynthia R. ^{[1
]}

Yuasa, Masato ^{[2
]}

Schoenecker, Jonathan ^{[2
,3
]}

Goudy, Steven L. ^{[1
,3
]}

机构：

[1] Vanderbilt Univ, Med Ctr, Dept Otolaryngol, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Med Ctr, Dept Orthoped, Nashville, TN 37232 USA

[3] Vanderbilt Univ, Med Ctr, Dept Pediat, Nashville, TN 37232 USA

来源：

BONE | 2014年 / 62卷

基金：

美国国家卫生研究院;

关键词：

Maxillary hypoplasia; Jagged1; Cranial neural crest; Mesenchymal cells; Osteoblasts; Ossification; CRANIAL NEURAL CREST; NOTCH; DIFFERENTIATION; ACTIVATION; INDUCTION; WNT;

D O I：

10.1016/j.bone.2014.01.019

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Maxillary hypoplasia occurs due to insufficient maxillary intramembranous ossification, leading to poor dental occlusion, respiratory obstruction and cosmetic deformities. Conditional deletion of Jagged1 (Jag1) in cranial neural crest (CNC) cells using Wnt1-cre; Jagged1(f/f) (Jag1CKO) led to maxillary hypoplasia characterized by intrinsic differences in bone morphology and density using mu CT evaluation. Jag1CKO maxillas revealed altered collagen deposition, delayed ossification, and reduced expression of early and late determinants of osteoblast development during maxillary ossification. In vitro bone cultures on Jag1CKO mouse embryonic maxillary mesenchymal (MEMM) cells demonstrated decreased mineralization that was also associated with diminished induction of osteoblast determinants. BMP receptor expression was dysregulated in the Jag1CKO MEMM cells suggesting that these cells were unable to respond to BMP-induced differentiation. JAG1-Fc rescued in vitro mineralization and osteoblast gene expression changes. These data suggest that JAG1 signaling in CNC-derived MEMM cells is required for osteoblast development and differentiation during maxillary ossification. (C) 2014 The Authors. Published by Elsevier Inc.

引用

页码：10 / 21

页数：12

共 31 条

[1]

HEPATIC DUCTULAR HYPOPLASIA ASSOCIATED WITH CHARACTERISTIC FACIES, VERTEBRAL MALFORMATIONS, RETARDED PHYSICAL, MENTAL AND SEXUAL DEVELOPMENT, AND CARDIAC MURMUR [J].