Conformational and biochemical differences in the TCR•CD3 complex of CD8+ versus CD4+ mature lymphocytes revealed in the absence of CD3γ

被引:27
作者
Zapata, DA
Pacheco-Castro, A
Torres, PS
Ramiro, AR
San José, E
Alarcón, B
Alibaud, L
Rubin, B
Toribio, ML
Regueiro, JR [1 ]
机构
[1] Univ Complutense Madrid, Fac Med, Madrid 28040, Spain
[2] Univ Autonoma Madrid, Fac Biol, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
[3] CHU Purpan, CNRS, Ctr Immunopathol Genet Humaine, F-31300 Toulouse, France
关键词
D O I
10.1074/jbc.274.49.35119
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mature CD4(+) and CD8(+) T lymphocytes are believed to build and express essentially identical surface alpha beta T-cell receptor-CD3 (TCR.CD3) complexes, However, TCR.CD3 expression has been shown to be more impaired in CD8(+) cells than in CD4(+) cells when CD3 gamma is absent in humans or mice. We have addressed this paradox by performing a detailed phenotypical and biochemical analysis of the TCR.CD3 complex in human CD3 gamma-deficient CD8+ and CD4(+) T cells. The results indicated that the membrane TCR.CD3 complex of CD8(+) T lymphocytes was conformationally different from that of CD4(+) lymphocytes in the absence of CD3 gamma, In addition, CD8(+), but not CD4(+), CD3 gamma-deficient T lymphocytes were shown to contain abnormally glycosylated TCR beta proteins, together with a smaller, abnormal TCR chain (probably incompletely processed TCR alpha), These results suggest the existence of hitherto unrecognized biochemical differences between mature CD4(+) and CD8(+) T lymphocytes in the intracellular control of alpha beta TCR.CD3 assembly, maturation, or transport that are revealed when CD3 gamma is absent, Such lineage-specific differences may be important in receptor-coreceptor interactions during antigen recognition.
引用
收藏
页码:35119 / 35128
页数:10
相关论文
共 39 条
[1]   FAMILIAL DEFECT IN THE SURFACE EXPRESSION OF THE T-CELL RECEPTOR CD3 COMPLEX [J].
ALARCON, B ;
REGUEIRO, JR ;
ARNAIZVILLENA, A ;
TERHORST, C .
NEW ENGLAND JOURNAL OF MEDICINE, 1988, 319 (18) :1203-1208
[2]   PRIMARY IMMUNODEFICIENCY CAUSED BY MUTATIONS IN THE GENE ENCODING THE CD3-GAMMA SUBUNIT OF THE LYMPHOCYTE-T RECEPTOR [J].
ARNAIZVILLENA, A ;
TIMON, M ;
CORELL, A ;
PEREZACIEGO, P ;
MARTINVILLA, JM ;
REGUEIRO, JR .
NEW ENGLAND JOURNAL OF MEDICINE, 1992, 327 (08) :529-533
[3]  
ARNAIZVILLENA A, 1991, LAB INVEST, V64, P675
[4]   The interchain disulfide bond between TCR alpha beta heterodimers on human T cells is not required for TCR-CD3 membrane expression and signal transduction [J].
Arnaud, J ;
Huchenq, A ;
Vernhes, MC ;
CasparBauguil, S ;
Lenfant, F ;
Sancho, J ;
Terhorst, C ;
Rubin, B .
INTERNATIONAL IMMUNOLOGY, 1997, 9 (04) :615-626
[5]   Mutation in splicing consensus sequences causes lack of TCR membrane expression due to exon excision [J].
Arnaud, J ;
Cayrou, C ;
Llobera, R ;
Rubin, B .
IMMUNOGENETICS, 1997, 45 (05) :311-319
[6]   IMMUNOCHEMICAL PROOF THAT A NOVEL REARRANGING GENE ENCODES THE T-CELL RECEPTOR-DELTA SUBUNIT [J].
BAND, H ;
HOCHSTENBACH, F ;
MCLEAN, J ;
HATA, S ;
KRANGEL, MS ;
BRENNER, MB .
SCIENCE, 1987, 238 (4827) :682-684
[7]   STABLE GROWTH TRANSFORMATION OF HUMAN LYMPHOCYTES-T BY HERPESVIRUS SAIMIRI [J].
BIESINGER, B ;
MULLERFLECKENSTEIN, I ;
SIMMER, B ;
LANG, G ;
WITTMANN, S ;
PLATZER, E ;
DESROSIERS, RC ;
FLECKENSTEIN, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :3116-3119
[8]   NON-RANDOM EXPRESSION OF T-CELL RECEPTOR-GAMMA AND RECEPTOR-DELTA VARIABLE GENE SEGMENTS IN FUNCTIONAL LYMPHOCYTE-T CLONES FROM HUMAN PERIPHERAL-BLOOD [J].
BORST, J ;
WICHERINK, A ;
VANDONGEN, JJM ;
DEVRIES, E ;
COMANSBITTER, WM ;
WASSENAAR, F ;
VANDENELSEN, P .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (09) :1559-1568
[9]   DISTINCT MOLECULAR-FORMS OF HUMAN T-CELL RECEPTOR GAMMA-DELTA DETECTED ON VIABLE T-CELLS BY A MONOCLONAL-ANTIBODY [J].
BORST, J ;
VANDONGEN, JJM ;
BOLHUIS, RLH ;
PETERS, PJ ;
HAFLER, DA ;
DEVRIES, E ;
VANDEGRIEND, RJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (05) :1625-1644
[10]   INTERACTION OF STAPHYLOCOCCUS-AUREUS TOXIN SUPERANTIGENS WITH HUMAN T-CELLS [J].
CHOI, YW ;
KOTZIN, B ;
HERRON, L ;
CALLAHAN, J ;
MARRACK, P ;
KAPPLER, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8941-8945