Streptozotocin diabetes and the expression of GLUT1 at the brush border and basolateral membranes of intestinal enterocytes

被引:28
作者
Boyer, S
Sharp, PA
Debnam, ES
Baldwin, SA
Srai, SKS
机构
[1] ROYAL FREE HOSP, SCH MED, DEPT PHYSIOL, LONDON NW3 2PF, ENGLAND
[2] ROYAL FREE HOSP, SCH MED, DEPT BIOCHEM & MOL BIOL, LONDON NW3 2PF, ENGLAND
[3] UNIV LEEDS, DEPT BIOCHEM & MOL BIOL, LEEDS LS2 9JT, W YORKSHIRE, ENGLAND
来源
FEBS LETTERS | 1996年 / 396卷 / 2-3期
基金
英国惠康基金;
关键词
sugar transport; GLUT1; transporter; diabetes mellitus; intestinal glucose uptake; brush border membrane; basolateral membrane; insulin;
D O I
10.1016/0014-5793(96)01102-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Changes in membrane expression of sodium-dependent glucose transporter (SGLT1) and glucose transporter isoform (GLUT2) protein have been implicated in the increased intestinal glucose transport in streptozotocin-diabetes. The possible involvement of GLUT1 in the transport response, however, has not previously been studied. Using confocal microscopy on tissue sections and Western blotting of purified brush border membrane (BBM) and basolateral membrane (BLM), we have examined enterocyte expression of GLUT1 in untreated and in 1 and 21 day streptozotocin diabetic rats. In control enterocytes, GLUT1 was absent at the BBM and detected at low levels at the BLM. Diabetes resulted in a 4- to 5-fold increased expression of GLUT1 at the BLM and the protein could also be readily detected at the BBM. Insulin treatment of diabetic rats increased GLUT1 level at the BBM but,vas without effect on expression of the protein at the BLM.
引用
收藏
页码:218 / 222
页数:5
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