Characterization of the basal inhibitor of class II transcription NC2 from Saccharomyces cerevisiae

被引：51

作者：

Goppelt, A ^{[1
]}

Meisterernst, M ^{[1
]}

机构：

[1] UNIV MUNICH,MOL BIOL LAB,GENZENTRUM,D-81377 MUNICH,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 22期

关键词：

D O I：

10.1093/nar/24.22.4450

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human NC2 utilizes a unique mechanism of repression of transcription by associating with TBP and inhibition of preinitiation complex formation, Here we have cloned two genes from Saccharomyces cerevisiae and functionally characterized them as yeast NC2. We show that yeast NC2 binds to TBP as a heterodimer and represses RNA polymerase II transcription during assembly of the preinitiation complex. Yeast NC2 is highly homologous to its human counterpart within histone fold domains. C-Terminal regions previously discussed to be important for repression in man are in part not conserved. The human alpha but not the beta subunit efficiently heterodimerizes and represses transcription in combination with the corresponding yeast subunit, Yeast and human NC2 inhibit transcription in the presence of yeast and human TBP. However, repression is optimal within one species. The N-terminus of human TBP supports repression of transcription by human but not by yeast NC2.

引用

页码：4450 / 4455

页数：6

共 33 条

[1] TOPOGRAPHY OF THE HISTONE OCTAMER SURFACE - REPEATING STRUCTURAL MOTIFS UTILIZED IN THE DOCKING OF NUCLEOSOMAL DNA [J].

ARENTS, G ;

MOUDRIANAKIS, EN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (22) :10489-10493

[2] THE HISTONE FOLD - A UBIQUITOUS ARCHITECTURAL MOTIF UTILIZED IN DNA COMPACTION AND PROTEIN DIMERIZATION [J].

ARENTS, G ;

MOUDRIANAKIS, EN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :11170-11174

[3] THE NUCLEOSOMAL CORE HISTONE OCTAMER AT 3.1-A RESOLUTION - A TRIPARTITE PROTEIN ASSEMBLY AND A LEFT-HANDED SUPERHELIX [J].

ARENTS, G ;

BURLINGAME, RW ;

WANG, BC ;

LOVE, WE ;

MOUDRIANAKIS, EN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10148-10152

[4] MOT1, A GLOBAL REPRESSOR OF RNA-POLYMERASE-II TRANSCRIPTION, INHIBITS TBP BINDING TO DNA BY AN ATP-DEPENDENT MECHANISM [J].

AUBLE, DT ;

HANSEN, KE ;

MUELLER, CGF ;

LANE, WS ;

THORNER, J ;

HAHN, S .

GENES & DEVELOPMENT, 1994, 8 (16) :1920-1934

[5] SOLUTION STRUCTURE OF THE C-TERMINAL CORE DOMAIN OF HUMAN TFIIB - SIMILARITY TO CYCLIN-A AND INTERACTION WITH TATA-BINDING PROTEIN [J].

BAGBY, S ;

KIM, SJ ;

MALDONADO, E ;

TONG, KI ;

REINBERG, D ;

IKURA, M .

CELL, 1995, 82 (05) :857-867

[6] A VARIETY OF DNA-BINDING AND MULTIMERIC PROTEINS CONTAIN THE HISTONE FOLD MOTIF [J].

BAXEVANIS, AD ;

ARENTS, G ;

MOUDRIANAKIS, EN ;

LANDSMAN, D .

NUCLEIC ACIDS RESEARCH, 1995, 23 (14) :2685-2691

[7] IDENTIFICATION AND CHARACTERIZATION OF DROSOPHILA RELATIVES OF THE YEAST TRANSCRIPTIONAL ACTIVATOR SNF2/SWI2 [J].

ELFRING, LK ;

DEURING, R ;

MCCALLUM, CM ;

PETERSON, CL ;

TAMKUN, JW .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (04) :2225-2234

[8] Crystal structure of the yeast TFIIA/TBP/DNA complex [J].

Geiger, JH ;

Hahn, S ;

Lee, S ;

Sigler, PB .

SCIENCE, 1996, 272 (5263) :830-836

[9] A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains [J].

Goppelt, A ;

Stelzer, G ;

Lottspeich, F ;

Meisterernst, M .

EMBO JOURNAL, 1996, 15 (12) :3105-3116

[10] IDENTIFICATION OF A YEAST PROTEIN HOMOLOGOUS IN FUNCTION TO THE MAMMALIAN GENERAL TRANSCRIPTION FACTOR, TFIIA [J].

HAHN, S ;

BURATOWSKI, S ;

SHARP, PA ;

GUARENTE, L .

EMBO JOURNAL, 1989, 8 (11) :3379-3382

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