Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks

被引:15
作者
Carnevale, S
Velásquez, JN
Labbé, JH
Chertcoff, A
Cabrera, MG
Rodríguez, MI
机构
[1] Inst Nacl Microbiol Dr Carlos G Malbran, Dept Parasitol, Buenos Aires, DF, Argentina
[2] Hosp Municipal Infecciosas Dr Francisco J Muniz, Buenos Aires, DF, Argentina
关键词
D O I
10.1128/CDLI.7.3.504-506.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.
引用
收藏
页码:504 / 506
页数:3
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