Evaluation of carbon tetrachloride-induced stress on rat hepatocytes by 31P NMR and MALDI-TOF mass spectrometry: lysophosphatidylcholine generation from unsaturated phosphatidylcholines

被引：15

作者：

Bauer, Alexander ^{[3
]}

Schumann, Anika ^{[2
]}

Gilbert, Matthias ^{[2
]}

Wilhelm, Christian ^{[2
]}

Hengstler, Jan G. ^{[3
]}

Schiller, Juergen ^{[1
]}

Fuchs, Beate ^{[1
]}

机构：

[1] Univ Leipzig, Inst Med Phys & Biophys, Fak Med, D-04107 Leipzig, Germany

[2] Univ Leipzig, Inst Biol 1, Dept Plant Physiol, D-04107 Leipzig, Germany

[3] Leibniz Res Ctr Working Environm & Human Factors, Dortmund, Germany

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2009年 / 159卷 / 01期

关键词：

P-31 NMR spectroscopy; MALDI-TOF MS; Carbon tetrachloride; Rat hepatocytes; Oxidative stress; Phosphatidylcholine; Lysophosphatidylcholine; OXIDATION-PRODUCTS; COLORIMETRIC ASSAY; LIPID-PEROXIDATION; DRUG-METABOLISM; HUMAN PLASMA; FATTY-ACIDS; LIVER; PHOSPHOLIPIDS; INDUCTION; MECHANISM;

D O I：

10.1016/j.chemphyslip.2009.02.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbon tetrachloride (CCl4) represents an excellent model to study oxidative injury of cells. It is widely accepted that hepatocellular injury is a consequence of the metabolic conversion of CCl4 into highly reactive, free radical intermediates. Among the direct toxic effects of CCl4, stimulation of lipid peroxidation and the binding of the electrophilic radicals to membrane lipids have been suggested to play important roles in the pathogenesis of irreversible cell damage. CCl4-induced liver damage was modeled in cultures of rat hepatocytes with the focus on alterations of phosphatidylcholine (PC). The PC acyl chain composition was analyzed by P-31 NMR spectroscopy and MALDI-TOF mass spectrometry. The content of the membrane arachidonoyl PC was decreased by almost 30% after incubation of the cells with CCl4. This relative decrease was found to correlate with increased concentrations of the corresponding saturated lysophosphatidylcholine (LPC). It is concluded that LPC represents a useful biomarker of CCl4-mediated damaging of hepatocytes. It is also speculated that de novo biosynthesis of PC is influenced by CCl4. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：21 / 29

页数：9

共 57 条

[1] Structural analysis of phosphatidylcholines by post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].

Al-Saad, KA ;

Siems, WF ;

Hill, HH ;

Zabrouskov, V ;

Knowles, NR .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2003, 14 (04) :373-382

[2] Formation of lysophospholipids from unsaturated phosphatidylcholines under the influence of hypochlorous acid [J].

Arnhold, J ;

Osipov, AN ;

Spalteholz, H ;

Panasenko, OM ;

Schiller, J .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1572 (01) :91-100

[3] Isolation of esterified fatty acids bound to serum albumin purified from human plasma and characterised by MALDI mass spectrometry [J].

Belgacem, Omar ;

Stubiger, Gerald ;

Allmaier, Guenter ;

Buchacher, Andrea ;

Pock, Katharina .

BIOLOGICALS, 2007, 35 (01) :43-49

[4]

Boll M, 2001, Z NATURFORSCH C, V56, P649

[5]

Boll M, 2001, Z NATURFORSCH C, V56, P111

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7]

BRITT SG, 1987, FASEB J, V46, P958

[8] Metabolic pathways of 4-bromo-2,5-dimethoxyphenethylamine (2C-B): analysis of phase I metabolism with hepatocytes of six species including human [J].

Carmo, H ;

Hengstler, JG ;

de Boer, D ;

Ringel, M ;

Remiao, F ;

Carvalho, F ;

Fernandes, E ;

dos Reys, LA ;

Oesch, F ;

Bastos, MD .

TOXICOLOGY, 2005, 206 (01) :75-89

[9] Reactions of N-methyl-2-phenylindole with malondialdehyde and 4-hydroxyalkenals.: Mechanistic aspects of the colorimetric assay of lipid peroxidation [J].

Erdelmeier, I ;

Gérard-Monnier, D ;

Yadan, JC ;

Chaudière, J .

CHEMICAL RESEARCH IN TOXICOLOGY, 1998, 11 (10) :1184-1194

[10] Alternative approaches for the detection of various phospholipid classes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].

Estrada, R ;

Yappert, MC .

JOURNAL OF MASS SPECTROMETRY, 2004, 39 (04) :412-422

← 1 2 3 4 5 6 →