On-line identification of further flavone C- and O-glycosides from sugarcane (Soccharum officinarum L., Gramineae) by HPLC-UV-MS

被引：59

作者：

Colombo, Renata

Yariwake, Janete H. ^{[1
]}

Queiroz, Emerson F.

Ndjoko, Karine

Hostettmann, Kurt

机构：

[1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP, Brazil

[2] Univ Geneva, Ecole Pharm Geneve Lausanne, Lab Pharmacognosie & Phytochim, Geneva, Switzerland

来源：

PHYTOCHEMICAL ANALYSIS | 2006年 / 17卷 / 05期

关键词：

HPLC-UV-MS; UV shift reagents; flavone glycosides; Saccharum officinarum L. (Gramineae);

D O I：

10.1002/pca.923

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

HPLC-UV-MS (APCI-MS/MS and CID/MS) was utilised for the identification of eight additional flavone glycosides from sugarcane (Saccharum officinarum L., Gramineae) extracts of bagasse, leaves and juice ('garapa'). Relevant information about substitution patterns was obtained through UV detection using post-column addition of shift reagents, while tandem MS provided structural information confirming the proposed structures of the C-glycosides vitexin, orientin, luteolin-8-C-(rhamnosylglucoside), 4',5'-dimethyl-luteolin-8-C-glycoside and the isomeric pair schaftoside-isoschaftoside, besides the O-glycosides tricin-7-O-neohesperidoside and tricin-7-O-glycoside. Copyright (c) 2006 John Wiley & Sons, Ltd.

引用

页码：337 / 343

页数：7

共 19 条

[1]

AMADO E, 1997, P EUR COST CONC ACT

[2]

Determination of flavonoids in cultivated sugarcane leaves, bagasse, juice and in transgenic sugarcane by liquid chromatography-UV detection [J].