DNA microarray analysis of differentially expressed genes responsive to bisphenol A, an alkylphenol derivative, in an in vitro mouse Sertoli cell model

被引：16

作者：

Tabuchi, Y

Zhao, QL

Kondo, T

机构：

[1] Toyama Med & Pharmaceut Univ, Fac Med, Mol Genet Res Ctr, Toyama 9300194, Japan

[2] Toyama Med & Pharmaceut Univ, Fac Med, Dept Radiol Sci, Toyama 9300194, Japan

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 2002年 / 89卷 / 04期

关键词：

bisphenol A; microarray; Sertoli cell;

D O I：

10.1254/jjp.89.413

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

To identify genes elicited by bisphenol A (BPA) in Sertoli cells, we carried out a microarray analysis of TTE3 cells (a mouse Sertoli cell line) treated with BPA. BPA (100, 200 and 400 muM) induced cell death concentration-dependently, with levels being 25%, 33% and 96%, respectively. Of the 1,081 genes analyzed, 3 genes showed decreased levels of expression while the remaining 10 genes showed increased levels in the cells treated with a subtoxic dose of BPA (200 muM). The expressions of six genes were confirmed by the TaqMan assay. These findings suggest that DNA microarray analysis is a useful tool for investigating the molecular mechanisms of the toxic effects of BPA in testicular cells.

引用

页码：413 / 416

页数：4

共 18 条

[1] Bisphenol-A differently affects estrogen receptors-α in estrous-cycling and lactating female rats [J].