Biotemplated nanopatterning of planar surfaces with molecular motors

被引：46

作者：

Reuther, Cordula

Hajdo, Lukasz

Tucker, Robert

Kasprzak, Andrzej A.

Diez, Stefan

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

[2] M Nencki Inst Expt Biol, PL-02093 Warsaw, Poland

[3] Univ Florida, Dept Mat Sci & Engn, Gainesville, FL 32611 USA

来源：

NANO LETTERS | 2006年 / 6卷 / 10期

关键词：

D O I：

10.1021/nl060922l

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report on the generation of nanometer-wide, non-topographical patterns of proteins on planar surfaces. In particular, we used the regular lattice of reconstituted microtubules as template structures to specifically bind and transfer kinesin-1 and nonclaret disjunctional motor proteins. The generated tracks, which comprise dense and structurally oriented arrays of functional motor proteins, proved to be highly efficient for the guiding of microtubule transporters.

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页码：2177 / 2183

页数：7

相关论文

共 31 条

[1]

ANDRADE JD, 1986, ADV POLYM SCI, V79, P1

[2] Assembly and transport of nanocrystal CdSe quantum dot nanocomposites using microtubules and kinesin motor proteins [J].

Bachand, GD ;

Rivera, SB ;

Boal, AK ;

Gaudioso, J ;

Liu, J ;

Bunker, BC .

NANO LETTERS, 2004, 4 (05) :817-821

[3] TM-AFM threshold analysis of macromolecular orientation: A study of the orientation of IgG and IgE on mica surfaces [J].

Bergkvist, M ;

Carlsson, J ;

Karlsson, T ;

Oscarsson, S .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1998, 206 (02) :475-481

[4] FAILURE OF A SINGLE-HEADED KINESIN TO TRACK PARALLEL TO MICROTUBULE PROTOFILAMENTS [J].

BERLINER, E ;

YOUNG, EC ;

ANDERSON, K ;

MAHTANI, HK ;

GELLES, J .

NATURE, 1995, 373 (6516) :718-721

[5] Conformation of microcontact-printed proteins by atomic force miroscopy molecular sizing [J].

Biasco, A ;

Pisignano, D ;

Krebs, B ;

Pompa, PP ;

Persano, L ;

Cingolani, R ;

Rinaldi, R .

LANGMUIR, 2005, 21 (11) :5154-5158

[6] Motor protein-driven unidirectional transport of micrometer-sized cargoes across isopolar microtubule arrays [J].

Böhm, KJ ;

Stracke, R ;

Mühlig, P ;

Unger, E .

NANOTECHNOLOGY, 2001, 12 (03) :238-244

[7]

BRACEWELL R, 1999, FOURIER TRANSFORM IT, P25

[8] Mechanisms of microtubule guiding on microfabricated kinesin-coated surfaces:: Chemical and topographic surface patterns [J].

Clemmens, J ;

Hess, H ;

Lipscomb, R ;

Hanein, Y ;

Böhringer, KF ;

Matzke, CM ;

Bachand, GD ;

Bunker, BC ;

Vogel, V .

LANGMUIR, 2003, 19 (26) :10967-10974

[9] Analysis of microtubule guidance in open microfabricated channels coated with the motor protein kinesin [J].

Clemmens, J ;

Hess, H ;

Howard, J ;

Vogel, V .

LANGMUIR, 2003, 19 (05) :1738-1744

[10] Kinesin takes one 8-nm step for each ATP that it hydrolyzes [J].

Coy, DL ;

Wagenbach, M ;

Howard, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (06) :3667-3671

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