Duplicate zebrafish runx2 orthologues are expressed in developing skeletal elements

被引：89

作者：

Flores, MV

Tsang, VWK

Hu, WJ

Kalev-Zylinska, M

Postlethwait, J

Crosier, P

Crosier, K

Fisher, S

机构：

[1] Johns Hopkins Univ, Sch Med, Nathans McKusick Inst Genet Med, Dept Med, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Sch Med, Nathans McKusick Inst Genet Med, Dept Cell Biol, Baltimore, MD 21287 USA

[3] Univ Auckland, Sch Med Sci, Dept Mol Med & Pathol, Auckland 1, New Zealand

[4] Johns Hopkins Univ, Sch Med, Biochem Cellular & Mol Biol Grad Program, Baltimore, MD 21287 USA

[5] Univ Oregon, Inst Neurosci, Eugene, OR 97403 USA

来源：

GENE EXPRESSION PATTERNS | 2004年 / 4卷 / 05期

基金：

美国国家卫生研究院;

关键词：

zebrafish; Runx2; skeleton; cartilage; bone; chondrocyte; osteoblast;

D O I：

10.1016/j.modgep.2004.01.016

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The differentiation of cells in the vertebrate skeleton is controlled by a precise genetic program. One crucial regulatory gene in the pathway encodes the transcription factor Runx2, which in mouse is required for differentiation of all osteoblasts and the proper development of a subset of hypertrophic chondrocytes. To explore the differentiation of skeletogenic cells in the model organism zebrafish (Danio rerio), we have identified two orthologues of the mammalian gene, runx2a and runx2b. Both genes share sequence homology and gene structure with the mammalian genes, and map to regions of the zebrafish genome displaying conserved synteny with the region where the human gene is localized. While both genes are expressed in developing skeletal elements, they show evidence of partial divergence in expression pattern, possibly explaining why both orthologues have been retained through teleost evolution. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：573 / 581

页数：9

共 45 条

[1] Differential regulation of the two principal Runx2/Cbfa1 N-terminal isoforms in response to bone morphogenetic protein-2 during development of the osteoblast phenotype [J].