Design and Analysis of Single-Cell Sequencing Experiments

被引：390

作者：

Gruen, Dominic ^{[1
,2
,3
]}

van Oudenaarden, Alexander ^{[1
,2
]}

机构：

[1] Royal Netherlands Acad Arts & Sci, Hubrecht Inst KNAW, NL-3584 CT Utrecht, Netherlands

[2] Univ Med Ctr Utrecht, Canc Genom Netherlands, NL-3584 CT Utrecht, Netherlands

[3] Max Planck Inst Immunobiol & Epigenet, D-79108 Freiburg, Germany

来源：

CELL | 2015年 / 163卷 / 04期

关键词：

WHOLE GENOME AMPLIFICATION; MESSENGER-RNA-SEQ; GENE-EXPRESSION; NOISE; HETEROGENEITY; MAPS; RECOMBINATION; QUANTITIES; EVOLUTION; DYNAMICS;

D O I：

10.1016/j.cell.2015.10.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can elucidate the cell type composition of a sample. However, single-cell sequencing comes with major technical challenges and yields complex data output. In this Primer, we provide an overview of available methods and discuss experimental design and single-cell data analysis. We hope that these guidelines will enable a growing number of researchers to leverage the power of single-cell sequencing.

引用

页码：799 / 810

页数：12

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