Primer tRNA(3)(Lys) the viral genome exists in unextended and two-base extended forms within mature human immunodeficiency virus type 1

被引：55

作者：

Huang, Y

Wang, J

Shalom, A

Li, Z

Khorchid, A

Wainberg, MA

Kleiman, L

机构：

[1] MCGILL UNIV,JEWISH GEN HOSP,LADY DAVIS INST MED RES,MONTREAL,PQ H3T 1E2,CANADA

[2] MCGILL UNIV,JEWISH GEN HOSP,MCGILL AIDS CTR,MONTREAL,PQ H3T 1E2,CANADA

[3] MCGILL UNIV,DEPT MED,MONTREAL,PQ H3T 1E2,CANADA

[4] MCGILL UNIV,DEPT IMMUNOL & MICROBIOL,MONTREAL,PQ H3T 1E2,CANADA

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 01期

关键词：

D O I：

10.1128/JVI.71.1.726-728.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In human immunodeficiency virus type 1, tRNA(3)(Lys) is placed upon the primer binding site, where it serves as the primer for reverse transcription. We show herein that genomic placement occurs independently of precursor protein processing and that the pieced tRNA(3)(Lys) exists in two major forms: unextended or in a form extended by the first two DNA bases incorporated, C and T. The two-base extended form of the tRNA(3)(Lys) is absent in a protease-negative mutant, indicating a requirement for mature viral proteins to initiate reverse transcription within the virus.

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页码：726 / 728

页数：3

相关论文

共 14 条

[1] DNA FOUND IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PARTICLES MAY NOT BE REQUIRED FOR INFECTIVITY [J].

ARTS, EJ ;

MAK, J ;

KLEIMAN, L ;

WAINBERG, MA .

JOURNAL OF GENERAL VIROLOGY, 1994, 75 :1605-1613

[2] HIV-1 REVERSE-TRANSCRIPTASE SPECIFICALLY INTERACTS WITH THE ANTICODON DOMAIN OF ITS COGNATE PRIMER TRANSFER-RNA [J].

BARAT, C ;

LULLIEN, V ;

SCHATZ, O ;

KEITH, G ;

NUGEYRE, MT ;

GRUNINGERLEITCH, F ;

BARRESINOUSSI, F ;

LEGRICE, SFJ ;

DARLIX, JL .

EMBO JOURNAL, 1989, 8 (11) :3279-3285

[3] A DELETION MUTATION IN THE 5' PART OF THE POL GENE OF MOLONEY MURINE LEUKEMIA-VIRUS BLOCKS PROTEOLYTIC PROCESSING OF THE GAG AND POL POLYPROTEINS [J].

CRAWFORD, S ;

GOFF, SP .

JOURNAL OF VIROLOGY, 1985, 53 (03) :899-907

[4] VIRAL-RNA ANNEALING ACTIVITIES OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NUCLEOCAPSID PROTEIN REQUIRE ONLY PEPTIDE DOMAINS OUTSIDE THE ZINC FINGERS [J].

DEROCQUIGNY, H ;

GABUS, C ;

VINCENT, A ;

FOURNIEZALUSKI, MC ;

ROQUES, B ;

DARLIX, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6472-6476

[5] ROLE OF CAPSID PRECURSOR PROCESSING AND MYRISTOYLATION IN MORPHOGENESIS AND INFECTIVITY OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 [J].

GOTTLINGER, HG ;

SODROSKI, JG ;

HASELTINE, WA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5781-5785

[6] Effects of modifying the tRNA(3)(Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription [J].

Huang, Y ;

Shalom, A ;

Li, Z ;

Wang, J ;

Mak, J ;

Wainberg, MA ;

Kleiman, L .

JOURNAL OF VIROLOGY, 1996, 70 (07) :4700-4706

[7] INITIATION OF REVERSE TRANSCRIPTION OF HIV-1 - SECONDARY STRUCTURE OF THE HIV-1 RNA/TRNA(3)(LYS) (TEMPLATE/PRIMER) COMPLEX [J].

ISEL, C ;

EHRESMANN, C ;

KEITH, G ;

EHRESMANN, B ;

MARQUET, R .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 247 (02) :236-250

[8] Specific initiation and switch to elongation of human immunodeficiency virus type 1 reverse transcription require the post-transcriptional modifications of primer tRNA(3)(Lys) [J].

Isel, C ;

Lanchy, JM ;

LeGrice, SFJ ;

Ehresmann, C ;

Ehresmann, B ;

Marquet, R .

EMBO JOURNAL, 1996, 15 (04) :917-924

[9] IDENTIFICATION OF TRANSFER-RNAS INCORPORATED INTO WILD-TYPE AND MUTANT HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].

JIANG, M ;

MAK, J ;

LADHA, A ;

COHEN, E ;

KLEIN, M ;

ROVINSKI, B ;

KLEIMAN, L .

JOURNAL OF VIROLOGY, 1993, 67 (06) :3246-3253

[10] SMALL FINGER PROTEIN OF AVIAN AND MURINE RETROVIRUSES HAS NUCLEIC-ACID ANNEALING ACTIVITY AND POSITIONS THE REPLICATION PRIMER TRANSFER-RNA ONTO GENOMIC RNA [J].

PRATS, AC ;

SARIH, L ;

GABUS, C ;

LITVAK, S ;

KEITH, G ;

DARLIX, JL .

EMBO JOURNAL, 1988, 7 (06) :1777-1783