Effective Dissolved Oxygen Control Strategy for High-Cell-Density Cultures

被引：19

作者：

Carcamo, M. ^{[1
]}

Saa, P. A. ^{[1
]}

Torres, J. ^{[1
]}

Torres, S. ^{[3
]}

Mandujano, P. ^{[2
]}

Correa, J. R. P. ^{[3
]}

Agosin, E. ^{[1
,3
]}

机构：

[1] Pontificia Univ Catolica Chile, Santiago, Chile

[2] Centrovet, Santiago, Chile

[3] Pontificia Univ Catolica Chile, Chem & Bioproc Engn Dept, Santiago, Chile

来源：

IEEE LATIN AMERICA TRANSACTIONS | 2014年 / 12卷 / 03期

关键词：

control strategy; split-range; volumetric mass transfer coefficient; high-cell-density cultures; FED-BATCH CULTIVATION; ESCHERICHIA-COLI; FERMENTATIONS;

D O I：

10.1109/TLA.2014.6827863

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

Dissolved oxygen control is critical to ensure microorganisms' growth in high-cell-density cultures. Since biological oxygen consumption strongly depends on biomass concentration, high-cell-density cultures demand an increasing and continuous oxygen supply. This is a challenging control problem and one of the major limitations of this type of cultures. The aim of this paper is to provide a simple dissolved oxygen control strategy capable of achieving high biomass levels by manipulating three process variables: agitation, aeration and oxygenation. This strategy was developed using a split-range control scheme specially designed so that the controller action is linear with respect to the maximum transfer rate (k(L)a.DO*). The controller parameters were tuned through simulation and later implemented and validated in Escherichia coli fed-batch cultivations. The strategy presented here allowed reaching high biomass concentrations while maintaining oxygen concentration at an appropriate level. The implementation of this strategy in high-cell-density cultures will help improve the productivity of aerobic cultures.

引用

页码：389 / 394

页数：6

共 17 条

[1]

ANDERSSON L, 1994, FEMS MICROBIOL REV, V14, P39, DOI 10.1016/0168-6445(94)90011-6

[2]

Bae C.S., 1997, Biotechnol. Bioproc. Eng, V2, P27, DOI DOI 10.1007/BF02932459

[3] Linear calibration function of luminescence quenching-based optical sensor for trace oxygen analysis [J].