Fluorescence studies on PAMAM dendrimers interactions with bovine serum albumin

被引：265

作者：

Klajnert, B ^{[1
]}

Bryszewska, M ^{[1
]}

机构：

[1] Univ Lodz, Dept Gen Biophys, PL-90237 Lodz, Poland

来源：

BIOELECTROCHEMISTRY | 2002年 / 55卷 / 1-2期

关键词：

PAMAM dendrimers; bovine serum albumin; fluorescence quenching; Stern-Volmer plot; tryptophan;

D O I：

10.1016/S1567-5394(01)00170-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyamidoamine (PAMAM) dendrimers (generation 3.5 and 4) interaction with bovine serum albumin (BSA) was studied. The intensity of intrinsic fluorescence of two tryptophan residues and a shift in wavelength of their emission maxima were chosen as indicators of protein conformational changes. It is shown that the generation 4 has a greater impact on spectral properties of serum albumin than generation 3.5. (C) 2002 Elsevier Science B.V All rights reserved.

引用

页码：33 / 35

页数：3

共 8 条

[1] Glucose and free radicals impair the antioxidant properties of serum albumin [J].