Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae

被引：43

作者：

Gubala, Aneta J. ^{[1
]}

Proll, David F. ^{[1
]}

机构：

[1] Def Sci & Technol Org, Human Protect & Performance Div, Melbourne, Vic, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 09期

关键词：

D O I：

10.1128/AEM.02597-05

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.

引用

页码：6424 / 6428

页数：5

共 28 条

[1] Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR
Albert, MJ
Islam, D
Nahar, S
Qadri, F
Falklind, S
Weintraub, A
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (06) : 1633 - 1635
[2] Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water
Aridgides, LJ
Doblin, MA
Berke, T
Dobbs, FC
Matson, DO
Drake, LA
[J]. MARINE POLLUTION BULLETIN, 2004, 48 (11-12) : 1096 - 1101
[3] Virulence genes in environmental strains of Vibrio cholerae
Chakraborty, S
Mukhopadhyay, AK
Bhadra, RK
Ghosh, AN
Mitra, R
Shimada, T
Yamasaki, S
Faruque, SM
Takeda, Y
Colwell, RR
Nair, GB
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (09) : 4022 - 4028
[4] Detection of RTX toxin gene in Vibrio cholerae by PCR
Chow, KH
Ng, TK
Yuen, KY
Yam, WC
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (07) : 2594 - 2597
[5] CHOWDHURY MAR, 1994, FEMS MICROBIOL LETT, V119, P377, DOI 10.1016/0378-1097(94)90443-X
[6] A collagenase-targeted multiplex PCR assay for identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus
Di Pinto, A
Ciccarese, G
Tantillo, G
Catalano, D
Forte, VT
[J]. JOURNAL OF FOOD PROTECTION, 2005, 68 (01) : 150 - 153
[7] Pathogenic potential of environmental Vibrio cholerae strains carrying genetic variants of the toxin-coregulated pilus pathogenicity island
Faruque, SM
Kamruzzaman, M
Meraj, IM
Chowdhury, N
Nair, GB
Sack, RB
Colwell, RR
Sack, DA
[J]. INFECTION AND IMMUNITY, 2003, 71 (02) : 1020 - 1025
[8] USE OF POLYMERASE CHAIN-REACTION FOR DETECTION OF TOXIGENIC VIBRIO-CHOLERAE 01 STRAINS FROM THE LATIN-AMERICAN CHOLERA EPIDEMIC
FIELDS, PI
POPOVIC, T
WACHSMUTH, K
OLSVIK, O
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) : 2118 - 2121
[9] Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools
Fukushima, H
Tsunomori, Y
Seki, R
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (11) : 5134 - 5146
[10] Multiplex real-time PCR detection of Vibrio cholerae
Gubala, Aneta J.
[J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 65 (02) : 278 - 293

← 1 2 3 →