Sequence analysis of VP2 gene of canine parvovirus isolated from domestic dogs in Japan in 1999 and 2000

被引:20
作者
Doki, Miho
Fujita, Kentaro
Miura, Ryuichi
Yoneda, Misako
Ishikawa, Yoshihisa
Taneno, Akira
Kai, Chieko
机构
[1] Univ Tokyo, Lab Anim Res Ctr, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan
[2] Intervet KK, Cent Res Labs, Kasumigaura, Ibaraki 3000134, Japan
关键词
canine parvovirus; phylogenetic tree; VP2; gene;
D O I
10.1016/j.cimid.2006.05.001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Seven strains of canine parvovirus (CPV) were isolated from affected dogs in Japan between 1999 and 2000, and their VP2 genes were genetically analyzed. Comparison of the predicted amino acid sequences of VP2 suggested that three field isolates corresponded to CPV type 2a, while the other four to CPV type 2b. The phylogenetic tree constructed from the VP2 genes showed that the newly isolated strains are classified into the cluster consisting of recent Japanese and Taiwanese field isolates, which are distinct from Vietnamese isolates, United States Isolates, or classical CPV type 2. These results suggest that the CPV transmission occurred between Japan and Taiwan in 1990s, and the offspring are still circulating in both countries. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:199 / 206
页数:8
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