Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9

被引：22

作者：

Mitchell, Matthew ^{[1
]}

Nam, Hyun-Joo ^{[1
]}

Carter, Adam ^{[1
]}

McCall, Angela ^{[1
]}

Rence, Chelsea ^{[1
]}

Bennett, Antonette ^{[1
]}

Gurda, Brittney ^{[1
]}

McKenna, Robert ^{[1
]}

Porter, Mark ^{[2
,3
]}

Sakai, Yoshihisa ^{[2
,3
]}

Byrne, Barry J. ^{[2
,3
]}

Muzyczka, Nicholas ^{[2
,3
]}

Aslanidi, George ^{[4
]}

Zolotukhin, Sergei ^{[4
]}

Agbandje-McKenna, Mavis ^{[1
]}

机构：

[1] Univ Florida, Coll Med, McKnight Brain Inst, Struct Biol Ctr,Dept Biochem & Mol Biol, Gainesville, FL 32610 USA

[2] Univ Florida, Coll Med, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA

[3] Univ Florida, Coll Med, Powell Gene Therapy Ctr, Gainesville, FL 32610 USA

[4] Univ Florida, Coll Med, Div Cell & Mol Therapy, Dept Pediat, Gainesville, FL 32610 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2009年 / 65卷

基金：

美国国家卫生研究院;

关键词：

CENTRAL-NERVOUS-SYSTEM; HUMAN GENE-THERAPY; VIRAL VECTORS; TRANSDUCTION; CRYSTALLIZATION; SPECIFICITY; DELIVERY; REGIONS; TYPE-2;

D O I：

10.1107/S1744309109021460

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adeno-associated virus (AAV) serotype 9, which is under development for gene-delivery applications, shows significantly enhanced capsid-associated transduction efficiency in muscle compared with other AAV serotypes. With the aim of characterizing the structural determinants of this property, the purification, crystallization and preliminary X-ray crystallographic analyses of the AAV9 viral capsid are reported. The crystals diffracted X-rays to 2.8 angstrom resolution using synchrotron radiation and belonged to the trigonal space group P3(2), with unit-cell parameters a = b = 251.0, c = 640.0 angstrom. There are three complete viral capsids in the crystal unit cell. The orientation and position of the asymmetric unit capsid have been determined by molecular-replacement methods and structure determination is in progress.

引用

页码：715 / 718

页数：4

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