Analysis of intracellular nucleotides by capillary electrophoresis -: Mass spectrometry

被引：6

作者：

Friedecky, D.

Bednar, P.

Prochazka, M.

Adam, T.

机构：

[1] Univ Hosp Olomouc, Lab Inherited Metabol Disorders, Dept Clin Biochem, Olomouc, Czech Republic

[2] Palacky Univ, Dept Analyt Chem, CR-77147 Olomouc, Czech Republic

[3] Univ Hosp Olomouc, Dept Obstet & Gynecol, Olomouc, Czech Republic

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2006年 / 25卷 / 9-11期

关键词：

capillary electrophoresis; mass spectrometry; metabolism; erythrocyte; purine disorders;

D O I：

10.1080/15257770600894667

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism - AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer ( 20 mmol/L, pH 4.4) and identified on line by mass spectrometry.

引用

页码：1233 / 1236

页数：4

共 3 条

[1] AICA-ribosiduria:: A novel, neurologically devastating inborn error of purine biosynthesis caused by mutation of ATIC [J].