The separation of polyphenols by isocratic hydrogen bond adsorption chromatography on a cross-linked 12% agarose gel

被引：25

作者：

Gu, M.

Su, Z. -G.

Janson, J. -C.

机构：

[1] Univ Uppsala, Uppsala Biomed Ctr, Dept Surface Biotechnol, S-75123 Uppsala, Sweden

[2] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China

来源：

CHROMATOGRAPHIA | 2006年 / 64卷 / 5-6期

基金：

中国国家自然科学基金;

关键词：

column liquid chromatography; hydrogen bond adsorption chromatography; polyphenols; agarose gel;

D O I：

10.1365/s10337-006-0039-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The highly cross-linked 12% agarose gel, Superose(TM) 12 HR 10/30 is shown to possess hydrogen bond acceptor properties suitable for the separation of polyphenolic solutes such as phenolic acids, flavonols and flavonoids. The separation is achieved isocratically in the presence of solvent mixtures of acetic acid and ethanol. The extent of hydrogen bond adsorption is reviewed based on data obtained from the elution behaviour of a variety of simple polyphenolic solutes including dihydroxybenzoic acids.

引用

页码：247 / 253

页数：7

共 14 条

[1]

AGAROSE-BASED MEDIA FOR HIGH-RESOLUTION GEL-FILTRATION OF BIO-POLYMERS [J].