New insights into water-phospholipid model membrane interactions

被引:185
作者
Milhaud, J [1 ]
机构
[1] Univ Paris 06, UMR CNRS 7033, Lab Physicochim Biomol & Cellulaire Chim & Spectr, F-75252 Paris 05, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2004年 / 1663卷 / 1-2期
关键词
phospholipid/water multibilayer; interdependence of water and phospholipid chemical potential; energetics of a phospholipid sample hydration; transmembrane water transport; properties of interfacial water; humidity-controlled osmotic stress;
D O I
10.1016/j.bbamem.2004.02.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Modulating the relative humidity (RH) of the ambient gas phase of a phospholipid/water sample for modifying the activity of phospholipid-sorbed water [humidity-controlled osmotic stress methods, J. Chem. Phys. 92 (1990) 4519 and J. Phys. Chem. 96 (1992) 446] has opened a new field of research of paramount importance. New types of phase transitions, occurring at specific values of this activity, have been then disclosed. Hence, it is become recognized that this activity, like the temperature T, is an intensive parameter of the thermodynamical state of these samples. This state can be therefore changed (phase transition) either, by modulating T at a given water activity (a given hydration level), or, by modulating the water activity, at a given T. The underlying mechanisms of these two types of transition differ, especially when they appear as disorderings of fatty chains. In lyotropic transitions, this disordering follows from two thermodynamical laws. First, acting on the activity (the chemical potential) of water external to a phospholipid/water sample, a transbilayer gradient of water chemical potential is created, leading to a transbilayer flux of water (Fick's law). Second, water molecules present within the hydrocarbon region of this phospholipid bilayer interact with phospholipid molecules through their chemical potential (Gibbs-Duhem relation): the conformational state of fatty chains (the thermodynamical state of the phospholipid molecules) changes. This process is slow, as revealed by osmotic stress time-resolved experiments. In thermal chain-melting transitions, the first rapid step is the disordering of fatty chains of a fraction of phospholipid molecules. It occurs a few degrees before the main transition temperature, T(m), during the pretransition and the sub-main transition. The second step, less rapid, is the redistribution of water molecules between the different parts of the sample, as revealed by T-jump time-resolved experiments. Finally, in lyotropic and thermal transitions, hydration and conformation are linked but the order of anteriority of their change, in each case, is probably not the same. In this review, first, the interactions of phospholipid submolecular fragments and water molecules, in the interfacial and hydrocarbon regions of phospholipid/water multibilayer stacks, will be described. Second, the coupling of the conformational states of phospholipid and water molecules, during thermal and lyotropic transitions, will be demonstrated through examples. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:19 / 51
页数:33
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