VNTR derived oligonucleotides as PCR primers for population studies in filamentous fungi

被引：25

作者：

Bridge, PD

Pearce, DA

Rivera, A

Rutherford, MA

机构：

[1] INT MYCOL INST,EGHAM TW20 9TY,SURREY,ENGLAND

[2] CTR NACL INVEST CAFE,MANIZALES,COLOMBIA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 24卷 / 05期

关键词：

D O I：

10.1046/j.1472-765X.1997.00060.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

One M13 phage derived and three simple sequence repeat primers were assessed for the development of a standard set of PCR primers for population studies with filamentous fungi. Fungal isolates from five plant or insect pathogenic genera with ascomycete and basidiomycete affinities were screened. Three of the four primers were found to be suitable for generating 'genetic fingerprints' for all of the fungal genera tested. Strain groupings obtained from the individual primers were found to be supportive through constrained two-dimensional cluster analysis.

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页码：426 / 430

页数：5

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