Transplantation of Embryonic and Induced Pluripotent Stem Cell-Derived 3D Retinal Sheets into Retinal Degenerative Mice

被引：287

作者：

Assawachananont, Juthaporn ^{[1
,4
]}

Mandai, Michiko ^{[1
]}

Okamoto, Satoshi ^{[1
,5
]}

Yamada, Chikako ^{[1
]}

Eiraku, Mototsugu ^{[2
]}

Yonemura, Shigenobu ^{[3
]}

Sasai, Yoshiki ^{[2
]}

Takahashi, Masayo ^{[1
]}

机构：

[1] RIKEN Ctr Dev Biol, Lab Retinal Regenerat, Kobe, Hyogo 6500047, Japan

[2] RIKEN Ctr Dev Biol, Organogenesis & Neurogenesis Grp, Kobe, Hyogo 6500047, Japan

[3] RIKEN Ctr Dev Biol, Electron Microscope Lab, Kobe, Hyogo 6500047, Japan

[4] Kyoto Univ, Grad Sch Med, Kyoto 6068501, Japan

[5] Keio Univ, Sch Med, Dept Physiol, Tokyo 1608582, Japan

来源：

STEM CELL REPORTS | 2014年 / 2卷 / 05期

关键词：

MOUSE RETINA; PHOTORECEPTOR TRANSPLANTATION; ROD PHOTORECEPTORS; VISUAL FUNCTION; GENERATION; RESTORATION; PRECURSORS; EXPRESSION; DIFFERENTIATION; EVOLUTION;

D O I：

10.1016/j.stemcr.2014.03.011

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

In this article, we show that mouse embryonic stem cell-or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. We also observed host-graft synaptic connections by immunohistochemistry. This study provides a "proof of concept'' for retinal sheet transplantation therapy for advanced retinal degenerative diseases.

引用

页码：662 / 674

页数：13

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