Real-Time Quantitative Reverse Transcription-PCR Assays Specifically Detecting Bluetongue Virus Serotypes 1, 6, and 8

被引：38

作者：

Hoffmann, Bernd ^{[1
]}

Eschbaumer, Michael ^{[1
]}

Beer, Martin ^{[1
]}

机构：

[1] Fridrich Loeffler Inst, Inst Diagnost Virol, Fed Res Inst Anim Hlth, D-17493 Greifswald, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 09期

关键词：

EPIDEMIOLOGY;

D O I：

10.1128/JCM.00599-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bluetongue virus (BTV) is a major pathogen of ruminants. Especially serotypes 1, 6, and 8 are of concern to veterinary authorities in central Europe. This article describes highly sensitive real-time reverse transcription-PCR assays directed to BTV genome segment 2 for specific detection of BTV-1, -6, or -8 in animal samples.

引用

收藏

页码：2992 / 2994

页数：3

相关论文

共 16 条

[1] Bluetongue virus: European Community inter-laboratory comparison tests to evaluate ELISA and RT-PCR detection methods [J].

Batten, C. A. ;

Bachanek-Bankowska, K. ;

Bin-Tarif, A. ;

Kgosana, L. ;

Swain, A. J. ;

Corteyn, M. ;

Darpel, K. ;

Mellor, P. S. ;

Elliott, H. G. ;

Oura, C. A. L. .

VETERINARY MICROBIOLOGY, 2008, 129 (1-2) :80-88

[2] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[3] Culicoides and the emergence of bluetongue virus in northern Europe [J].

Carpenter, Simon ;

Wilson, Anthony ;

Mellor, Philip S. .

TRENDS IN MICROBIOLOGY, 2009, 17 (04) :172-178

[4] Epidemiology of Bluetongue Virus Serotype 8, Germany [J].

Conraths, Franz J. ;

Gethmann, Joern M. ;

Staubach, Christoph ;

Mettenleiter, Thomas C. ;

Beer, Martin ;

Hoffmann, Bernd .

EMERGING INFECTIOUS DISEASES, 2009, 15 (03) :433-435

[5]

Hateley G, 2009, IN PRACTICE, V31, P202

[6]

HOFFMANN B, VET MICROBI IN PRESS

[7] Bluetongue virus serotype 8 reemergence in Germany, 2007 and 2008 [J].

Hoffmann, Bernd ;

Sasserath, Michael ;

Thalheim, Sabine ;

Bunzenthal, Claudia ;

Strebelow, Guenter ;

Beer, Martin .

EMERGING INFECTIOUS DISEASES, 2008, 14 (09) :1421-1423

[8] Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes [J].

Maan, S. ;

Maan, N. S. ;

Samuel, A. R. ;

Rao, S. ;

Attoui, H. ;

Mertens, P. P. C. .

JOURNAL OF GENERAL VIROLOGY, 2007, 88 :621-630

[9] Sequence analysis of bluetongue virus serotype 8 from the Netherlands 2006 and comparison to other European strains [J].

Maan, Sushila ;

Maan, Narender S. ;

Ross-Smith, Natalie ;

Batten, Carrie A. ;

Shaw, Andrew E. ;

Anthony, Simon J. ;

Samuel, Alan R. ;

Darpel, Karin E. ;

Veronesi, Eva ;

Oura, Chris A. L. ;

Singh, Karam P. ;

Nomikou, Kyriaki ;

Potgieter, Abraham C. ;

Attoui, Houssam ;

van Rooij, Eugene ;

van Rijn, Piet ;

De Clercq, Kris ;

Vandenbussche, Frank ;

Zientara, Stephan ;

Breard, Emmanuel ;

Sailleau, Corinne ;

Beer, Martin ;

Hoffman, Bernd ;

Mellor, Philip S. ;

Mertens, Peter P. C. .

VIROLOGY, 2008, 377 (02) :308-318

[10] The Pathology and Pathogenesis of Bluetongue [J].

Maclachlan, N. J. ;

Drew, C. P. ;

Darpel, K. E. ;

Worwa, G. .

JOURNAL OF COMPARATIVE PATHOLOGY, 2009, 141 (01) :1-16