First functional characterization of a singly expressed bacterial type II topoisomerase:: The enzyme from Mycohacterium wherculosis

被引：91

作者：

Aubry, Alexandra

Fisher, L. Mark

Jarlier, Vincent

Cambau, Ernmanuelle

机构：

[1] Univ Paris 06, EA1541, Fac Med Pitie Salpetriere, Lab Bacteriol, F-75252 Paris 05, France

[2] Univ London, Div Basic Med Sci, Mol Genet Grp, Mol & Metab Signalling Ctr, London SW17 0RE, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 348卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

topoisomerase; decatenation; Mycobacterium tuberculosis; DNA gyrase;

D O I：

10.1016/j.bbrc.2006.07.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genome deciphering revealed that Mycobacterium tuberculosis encodes a single type 11 topoisomerase contrary to common bacteria harboring two type II topoisomerases (DNA gyrase and topoisomerase IV). Functions of the M. tuberculosis type 11 topoisomerase were explored after cloning and expressing the subunits encoding genes in Escherichia coli. M. tuberculosis type 11 topoisomerase supercoiled relaxed pBR322 with a specific activity close to that of DNA gyrases of common bacteria whereas it exhibited DNA relaxation and formation of cleavable complexes with activities significantly higher than other DNA gyrases. Intermolecular passage activity evaluated by the decatenation of kinetoplast DNA was 25-fold lower than that of the topoisomerase IV from Streptococcus pneumoniae, but was markedly higher than that of the E coli gyrase. Overall, the type II topoisomerase of M. tuberculosis exhibits classical polyvalent activities of DNA gyrase for supercoiling but enhanced relaxation, cleavage, and decatenation activities. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：158 / 165

页数：8

共 28 条

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