Cloning and expression of human liver rhodanese cDNA

被引：13

作者：

Aita, N ^{[1
]}

Ishii, K ^{[1
]}

Akamatsu, Y ^{[1
]}

Ogasawara, Y ^{[1
]}

Tanabe, S ^{[1
]}

机构：

[1] MEIJI COLL PHARM,DEPT HYGIEN CHEM,TANASHI,TOKYO 188,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 231卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.6046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA for the human rhodanese (thiosulfate; cyanide sulfurtransferase, EC 2.8.1.1) was cloned from a human fetal liver cDNA library, Sequencing of the cDNA revealed an open reading frame that encodes a 297-residue polypeptide with a calculated mass of 33427 daltons. When the rhodanese cDNA was transientlly expressed in Escherichia coli and Cos7 cells, the rhodanese activity increased 40-fold and 150-fold, respectively, Sequence homology analysis showed that the human rhodanese is 89.6% identical to bovine, 90.2% identical to rat, 91.2% identical to mouse and Chinese hamster, and 71.4% similar to avian counterparts, respectively, and that rhodanese was highly conserved across evolution. (C) 1997 Academic Press.

引用

页码：56 / 60

页数：5

共 18 条

[1]

Agro A F., 1971, FEBS Lett, V16, P172, DOI 10.1016/0014-5793(71)80124-2

[2] MOUSE RHODANESE GENE (TST) - CDNA CLONING, SEQUENCING, AND RECOMBINANT PROTEIN EXPRESSION [J].

DOOLEY, TP ;

NAIR, SK ;

GARCIA, RE ;

COURTNEY, BC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 216 (03) :1101-1109

[3] CHROMOSOME ASSIGNMENT OF HUMAN BRAIN EXPRESSED SEQUENCE TAGS (ESTS) BY ANALYZING FLUORESCENTLY LABELED PCR PRODUCTS FROM HYBRID CELL PANELS [J].

DURKIN, AS ;

NIERMAN, WC ;

ZOGHBI, H ;

JONES, C ;

KOZAK, CA ;

MAGLOTT, DR .

CYTOGENETICS AND CELL GENETICS, 1994, 65 (1-2) :86-91

[4]

Jarabak R, 1981, Methods Enzymol, V77, P291

[5] PRIMARY STRUCTURE OF AVIAN HEPATIC RHODANESE [J].

KOHANSKI, RA ;

HEINRIKSON, RL .

JOURNAL OF PROTEIN CHEMISTRY, 1990, 9 (04) :369-377

[6] TOWARD A CDNA MAP OF THE HUMAN GENOME [J].

KORENBERG, JR ;

CHEN, XN ;

ADAMS, MD ;

VENTER, JC .

GENOMICS, 1995, 29 (02) :364-370

[7]

MILLER DM, 1991, J BIOL CHEM, V266, P486

[8]

Nishino T, 1986, Adv Exp Med Biol, V195 Pt B, P259

[9] REVERSIBLE INTERCONVERSION BETWEEN SULFO AND DESULFO XANTHINE-OXIDASE IN A SYSTEM CONTAINING RHODANESE, THIOSULFATE, AND SULFHYDRYL REAGENT [J].

NISHINO, T ;

USAMI, C ;

TSUSHIMA, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (07) :1826-1829

[10] CLONING AND SEQUENCE-ANALYSIS OF THE HUMAN LIVER RHODANESE - COMPARISON WITH THE BOVINE AND CHICKEN ENZYMES [J].

PALLINI, R ;

GUAZZI, GC ;

CANNELLA, C ;

CACACE, MG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 180 (02) :887-893

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