Enhanced production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture

被引：152

作者：

Ip, PF ^{[1
]}

Wong, KH ^{[1
]}

Chen, F ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2004年 / 39卷 / 11期

关键词：

Chlorella zofingiensis; astaxanthin; glucose; nitrate; mixotrophic culture;

D O I：

10.1016/j.procbio.2003.08.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mixotrophic culture may be used as an efficient means for enhanced production of light-induced pigments in microalgae. The growth and pigments formation of the green microalga Chlorella zofingiensis cultivated at various glucose and nitrate concentrations in mixotrophic culture were investigated. High glucose and low nitrate concentrations favoured the production of astaxanthin in the algal culture. The highest yield of astaxanthin was 12.5 mg l(-1), which was obtained in the medium containing 30 g l(-1) glucose and 0.55 g l(-1) nitrate. The results indicated that supplementation of glucose and nitrate in the culture medium could be effectively manipulated to enhance astaxanthin production by C. zofingiensis in mixotrophic culture. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1761 / 1766

页数：6

共 26 条

[1] PIGMENT AND STRUCTURAL-CHANGES IN CHLORELLA-ZOFINGIENSIS UPON LIGHT AND NITROGEN STRESS [J].

BAR, E ;

RISE, M ;

VISHKAUTSAN, M ;

ARAD, S .

JOURNAL OF PLANT PHYSIOLOGY, 1995, 146 (04) :527-534

[2] CULTURE OF THE ASTAXANTHIN-PRODUCING GREEN-ALGA HAEMATOCOCCUS-PLUVIALIS .1. EFFECTS OF NUTRIENTS ON GROWTH AND CELL TYPE [J].

BOROWITZKA, MA ;

HUISMAN, JM ;

OSBORN, A .

JOURNAL OF APPLIED PHYCOLOGY, 1991, 3 (04) :295-304

[3] ASTAXANTHIN ACCUMULATION IN THE GREEN-ALGA HAEMATOCOCCUS-PLUVIALIS [J].

BOUSSIBA, S ;

VONSHAK, A .

PLANT AND CELL PHYSIOLOGY, 1991, 32 (07) :1077-1082

[4]

BURICK P, 1991, BIORESOURCE TECHNOL, V38, P237

[5] SUBSTRATE-INHIBITION OF CHLAMYDOMONAS-REINHARDTII BY ACETATE IN HETEROTROPHIC CULTURE [J].

CHEN, F ;

JOHNS, MR .

PROCESS BIOCHEMISTRY, 1994, 29 (04) :245-252

[6] Relationship between substrate inhibition and maintenance energy of Chlamydomonas reinhardtii in heterotrophic culture [J].

Chen, F ;

Johns, MR .

JOURNAL OF APPLIED PHYCOLOGY, 1996, 8 (01) :15-19

[7] High cell density mixotrophic culture of Spirulina platensis on glucose for phycocyanin production using a fed-batch system [J].

Chen, F ;

Zhang, YM .

ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (03) :221-224

[8]

Darley WM, 1982, ALGAL BIOL PHYSL APP, P21

[9] Distribution of the mevalonate and glyceraldehyde phosphate/pyruvate pathways for isoprenoid biosynthesis in unicellular algae and the cyanobacterium Synechocystis PCC 6714 [J].

Disch, A ;

Schwender, J ;

Müller, C ;

Lichtenthaler, HK ;

Rohmer, M .

BIOCHEMICAL JOURNAL, 1998, 333 :381-388

[10] Extractability of astaxanthin in a mixed culture of a carotenoid over-producing mutant of Xanthophyllomyces dendrorhous and Bacillus circulans in two-stage batch fermentation [J].

Fang, TJ ;

Wang, JM .

PROCESS BIOCHEMISTRY, 2002, 37 (11) :1235-1245

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