Strand pairing by Rad54 and Rad51 is enhanced by chromatin

被引：127

作者：

Alexiadis, V ^{[1
]}

Kadonaga, JT ^{[1
]}

机构：

[1] Univ Calif San Diego, Mol Biol Sect, La Jolla, CA 92093 USA

来源：

GENES & DEVELOPMENT | 2002年 / 16卷 / 21期

关键词：

Rad54; Rad51; chromatin; homologous recombination;

D O I：

10.1101/gad.1032102

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated the role of chromatin in the catalysis of homologous strand pairing by Rad54 and Rad51. Rad54 is related to the ATPase subunits of chromatin-remodeling factors, whereas Rad51 is related to bacterial RecA. In the absence of superhelical tension, we found that the efficiency of strand pairing with chromatin is >100-fold higher than that with naked DNA. In addition, we observed that Rad54 and Rad51 function cooperatively in the ATP-dependent remodeling of chromatin. These findings indicate that Rad54 and Rad51 have evolved to function with chromatin, the natural substrate, rather than with naked DNA.

引用

页码：2767 / 2771

页数：5

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