Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS

被引:19
作者
Casabianca, A [1 ]
Orlandi, C [1 ]
Fraternale, A [1 ]
Magnani, M [1 ]
机构
[1] Univ Urbino, Inst Biol Chin Giorgio Fornaini, I-60129 Urbino, PU, Italy
关键词
D O I
10.1128/JCM.42.9.4361-4364.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy.
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页码:4361 / 4364
页数:4
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