The human Duffy antigen binds selected inflammatory but not homeostatic chemokines

被引：96

作者：

Gardner, L

Patterson, AM

Ashton, BA

Stone, MA

Middleton, J ^{[1
]}

机构：

[1] Keele Univ, Robert Jones & Agnes Hunt Orthopaed Hosp, Inst Sci & Technol Med, Leopold Muller Arthrit Res Ctr, Oswestry SY10 7AG, Shrops, England

[2] AstraZeneca, Resp & Inflammat Res, Macclesfield SK10 4TG, Cheshire, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 321卷 / 02期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

human Duffy antigen; selective chemokine binding; synovium; endothelium; erythrocytes;

D O I：

10.1016/j.bbrc.2004.06.146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the study was to compare the ability of the human Duffy antigen to bind homeostatic and inflammatory chemokines. Homeostatic chemokines did not bind to the Duffy antigen on erythrocytes with high affinity. in contrast, 60% of inflammatory chemokines bound strongly to Duffy, with no obvious preference for CXC or CC classes. It was investigated if this binding profile was reflected in the binding pattern of endothelial cells. Two examples of homeostatic (I-125-CXCL12 and I-125-CCL21) and inflammatory (I-125-CXCLS and I-125-CCL5) chemokines were incubated with human synovia. In agreement with the erythrocyte binding data, intense specific signals for CXCL8 and CCL5 binding were found on endothelial cells, whereas CXCL12 and CCL21 showed only weak binding to these cells. Our study provides evidence that the human Duffy antigen binds selected inflammatory, but not homeostatic, chemokines and that this binding pattern is reflected by endothelial cells within inflamed and non-inflamed tissue. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：306 / 312

页数：7

共 46 条

[1]

BEUTLER E, 1976, J LAB CLIN MED, V88, P328

[2] Stromal cell-derived factor 1 (CXCL12) induces monocyte migration into human synovium transplanted onto SCID mice [J].

Blades, MC ;

Ingegnoli, F ;

Wheller, SK ;

Manzo, A ;

Wahid, S ;

Panayi, GS ;

Perretti, M ;

Pitzalis, C .

ARTHRITIS AND RHEUMATISM, 2002, 46 (03) :824-836

[3]

BOZIC CR, 1994, J BIOL CHEM, V269, P29355

[4] Persistent induction of the chemokine receptor CXCR4 by TGF-β1 on synovial T cells contributes to their accumulation within the rheumatoid synovium [J].

Buckley, CD ;

Amft, N ;

Bradfield, PF ;

Pilling, D ;

Ross, E ;

Arenzana-Seisdedos, F ;

Amara, A ;

Curnow, SJ ;

Lord, JM ;

Scheel-Toellner, D ;

Salmon, M .

JOURNAL OF IMMUNOLOGY, 2000, 165 (06) :3423-3429

[5] Enhancing effect of IL-1, IL-17, and TNF-α on macrophage inflammatory protein-3α production in rheumatoid arthritis:: Regulation by soluble receptors and Th2 cytokines [J].

Chabaud, M ;

Page, G ;

Miossec, P .

JOURNAL OF IMMUNOLOGY, 2001, 167 (10) :6015-6020

[6] Detection of duffy antigen in the plasma membranes and caveolae of vascular endothelial and epithelial cells of nonerythroid organs [J].

Chaudhuri, A ;

Nielsen, S ;

Elkjaer, ML ;

Zbrzezna, V ;

Fang, F ;

Pogo, AO .

BLOOD, 1997, 89 (02) :701-712

[7]

CHAUDHURI A, 1994, J BIOL CHEM, V269, P7835

[8] Exaggerated response to endotoxin in mice lacking the Duffy antigen/receptor for chemokines (DARC) [J].

Dawson, TC ;

Lentsch, AB ;

Wang, ZX ;

Cowhig, JE ;

Rot, A ;

Maeda, N ;

Peiper, SC .

BLOOD, 2000, 96 (05) :1681-1684

[9] Targeted disruption of the beta-chemokine receptor CCR1 protects against pancreatitis-associated lung injury [J].

Gerard, C ;

Frossard, JL ;

Bhatia, M ;

Saluja, A ;

Gerard, NP ;

Lu, B ;

Steer, M .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (08) :2022-2027

[10] Heterogeneity of endothelial cells - The specialized phenotype of human high endothelial venules characterized by suppression subtractive hybridization [J].

Girard, JP ;

Baekkevold, ES ;

Yamanaka, T ;

Haraldsen, G ;

Brandtzaeg, P ;

Amalric, F .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (06) :2043-2055

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