Paraoxonase-1 inhibits oxidised LDL-induced MCP-1 production by endothelial cells

被引：160

作者：

Mackness, B ^{[1
]}

Hine, D ^{[1
]}

Liu, YF ^{[1
]}

Mastorikou, M ^{[1
]}

Mackness, M ^{[1
]}

机构：

[1] Manchester Royal Infirm, Dept Med, Manchester M13 9WL, Lancs, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 318卷 / 03期

关键词：

paraoxonase-1; HDL; MCP-1; oxidation; endothelial cells;

D O I：

10.1016/j.bbrc.2004.04.056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The upregulation of endothelial cell MCP-1 production by ox-LDL is a major initiating event in atherogenesis. HDL and PON1 retard the oxidation of LDL and therefore may retard endothelial cell MCP-1 production. The endothelial cell line EAhy926 was incubated with ox-LDL in the presence and absence of HDL and PON1 and the production of MCP-1 was measured by ELISA. Human HDL and PON1 significantly inhibited the in vitro oxidation of LDL and completely prevented the ox-LDL induced increase in MCP-1 production by endothelial cells. Ostrich HDL that does not contain PON1 was unable to prevent LDL-oxidation or the production of MCP-1 by endothelial cells. PON1 attenuates the ox-LDL induced MCP-1 production by endothelial cells. This is one, early, mechanism by which PON1 may be anti-atherogenic. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：680 / 683

页数：4

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