The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

被引：34

作者：

Kontula, P ^{[1
]}

Suihko, ML ^{[1
]}

Suortti, T ^{[1
]}

Tenkanen, M ^{[1
]}

Mattila-Sandholm, T ^{[1
]}

von Wright, A ^{[1
]}

机构：

[1] VTT Biotechnol & Food Res, FIN-02044 Espoo, Finland

来源：

FOOD MICROBIOLOGY | 2000年 / 17卷 / 01期

关键词：

D O I：

10.1006/fmic.1999.0268

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched cinder these conditions. From 161 isolates screened, 28% were identified by ribotyping as Lactobacillus rhamnosus, 29% as L, salivarius, 14% as L. cellobiosus, 13% as L, paracasei and the rest remained unidentified L, rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 and L, paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth. (C) 2000 Academic Press.

引用

页码：13 / 22

页数：10

共 20 条

[1]

Ballonge J, 1998, LACTIC ACID BACTERIA, P519

[2] Production, properties and applications of food-grade oligosaccharides [J].