Capillary monoliths for the analysis of nucleic acids by high-performance liquid chromatography-electrospray ionization mass spectrometry

被引：99

作者：

Oberacher, H

Huber, CG

机构：

[1] Univ Saarland, D-66041 Saarbrucken, Germany

[2] Univ Innsbruck, Inst Analyt Chem & Radiochem, A-6020 Innsbruck, Austria

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2002年 / 21卷 / 03期

基金：

奥地利科学基金会;

关键词：

nucleic acids; ohgonucleotides; ion-pair reversed-phase liquid chromatography; monolithic capillary columns; electrospray ionization-mass spectrometry (ESI-MS);

D O I：

10.1016/S0165-9936(02)00304-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monolithic capillary columns prepared by copolymerization of styrene and divinylbenzene inside a 200 mum i.d. fused silica capillary allow the rapid and highly efficient separation of single- and double-stranded DNA by ion-pair reversed-phase high-performance liquid chromatography. Hyphenation to electrospray ionization-mass spectrometry can be readily achieved. (C) 2002 Published by Elsevier Science B.V. All rights reserved.

引用

页码：166 / 174

页数：9

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