A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes

被引:44
作者
Kelkar, Samir
De, Bishnu P.
Gao, Guangping
Wilson, James M.
Crystal, Ronald G.
Leopold, Philip L.
机构
[1] Cornell Univ, Weill Med Coll, Dept Med Genet, New York, NY 10021 USA
[2] Cornell Univ, Weill Med Coll, Grad Program Physiol & Biophys & Syst Biol, New York, NY 10021 USA
[3] Univ Penn, Sch Med, Div Med Genet, Philadelphia, PA 19104 USA
关键词
D O I
10.1128/JVI.00481-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.
引用
收藏
页码:7781 / 7785
页数:5
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