Semiquantitative analysis of intrahepatic cytokine mRNAs in chronic hepatitis C

被引：48

作者：

Dumoulin, FL ^{[1
]}

Bach, A ^{[1
]}

Leifeld, L ^{[1
]}

ElBakri, M ^{[1
]}

Fischer, HP ^{[1
]}

Sauerbruch, T ^{[1
]}

Spengler, U ^{[1
]}

机构：

[1] UNIV BONN,INST PATHOL,D-53127 BONN,GERMANY

来源：

JOURNAL OF INFECTIOUS DISEASES | 1997年 / 175卷 / 03期

关键词：

D O I：

10.1093/infdis/175.3.681

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to characterize intrahepatic cytokine production, the mRNA levels of interleukin (IL)-2, -4, and -10 and interferon (IFN)-gamma were semiquantitatively determined by reverse-transcription competitive polymerase chain reaction in liver specimens from patients with chronic hepatitis C (n = 23), chronic hepatitis B (n = 9), or primary biliary cirrhosis (n = 12) and normal liver (control) specimens (n = 12). IL-4 mRNA was undetectable, Similar IL-10 mRNA levels were detected in all samples studied, including the controls. Mean IFN-gamma and IL-2 mRNA levels were elevated in chronic inflammatory liver disease. IL-2 mRNA levels were similar in all 3 patient groups, but intrahepatic IFN-gamma mRNA levels were significantly higher in chronic hepatitis C than in chronic hepatitis B or primary biliary cirrhosis patients. This predominance of IFN-gamma may indicate a lower susceptibility of hepatitis C virus to the antiviral effects of this cytokine. The presence of IL-10 in normal liver may impair the induction of antiviral immune responses.

引用

页码：681 / 685

页数：5

共 15 条

[1] TO C OR NOT TO C - THESE ARE THE QUESTIONS [J].