Automated sample mounting and alignment system for biological crystallography at a synchrotron source

被引：83

作者：

Snell, G ^{[1
]}

Cork, C

Nordmeyer, R

Cornell, E

Meigs, G

Yegian, D

Jaklevic, J

Jin, J

Stevens, RC

Earnest, T

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley Ctr Struct Biol, Phys Biosci Div, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Engn, Berkeley, CA 94720 USA

[3] Scripps Res Inst, Dept Biol Mol, La Jolla, CA 90237 USA

来源：

STRUCTURE | 2004年 / 12卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.str.2004.03.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-throughput data collection for macromolecular crystallography requires an automated sample mounting and alignment system for cryo-protected crystals that functions reliably when integrated into protein-crystallography beamlines at synchrotrons. Rapid mounting and dismounting of the samples increases the efficiency of the crystal screening and data collection processes, where many crystals can be tested for the quality of diffraction. The sample-mounting subsystem has random access to 112 samples, stored under liquid nitrogen. Results of extensive tests regarding the performance and reliability of the system are presented. To further increase throughput, we have also developed a sample transport/storage system based on "puck-shaped" cassettes, which can hold sixteen samples each. Seven cassettes fit into a standard dry shipping Dewar. The capabilities of a robotic crystal mounting and alignment system with instrumentation control software and a relational database allows for automated screening and data collection to be developed.

引用

收藏

页码：537 / 545

页数：9

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