Diagnosis of pulmonary infection with Toxoplasma gondii in immunocompromised HIV-positive patients by real-time PCR

被引：32

作者：

Petersen, E.

Edvinsson, B.

Lundgren, B.

Benfield, T.

Evengard, B. ^{[1
]}

机构：

[1] Karolinska Univ Hosp Huddinge, Karolinska Inst, Dept Lab Med F82, S-14186 Huddinge, Sweden

[2] Karolinska Univ Hosp Huddinge, Karolinska Inst, Dept Clin Bacteriol F82, S-14186 Huddinge, Sweden

[3] Aarhus Univ Hosp Skejby, Dept Infect Dis, DK-8200 Aarhus N, Denmark

[4] Hvidovre Univ Hosp Hvidovre, Dept Infect Dis, DK-2650 Hvidovre, Denmark

[5] Hvidovre Univ Hosp Hvidovre, Copenhagen HIV Programme, DK-2650 Hvidovre, Denmark

[6] Hvidovre Univ Hosp Hvidovre, Dept Clin Microbiol, DK-2650 Hvidovre, Denmark

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2006年 / 25卷 / 06期

关键词：

D O I：

10.1007/s10096-006-0156-5

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of the study presented here was to evaluate the use of PCR for improving the diagnosis of Toxoplasma gondii infection in immunocompromised hosts. Three hundred thirty-two bronchoalveolar lavage (BAL) fluid samples were analyzed by real-time PCR targeting a 529 bp element of T. gondii. In positive samples, the genotype of the parasite was determined by sequence analysis of the GRA6 gene. Positive results were achieved for 2% (7/332) of the samples tested. Genotyping was possible in two samples and revealed GRA6 type II T. gondii. PCR for detecting T. gondii in BAL samples should be performed in all immunosuppressed HIV-positive patients with symptoms of a systemic infection of unknown etiology. Trimethoprim-sulfamethoxazole prophylaxis does not exclude concomitant infection with T. gondii.

引用

页码：401 / 404

页数：4

共 27 条

[1] PCR for diagnosis and follow-up of two cases of disseminated toxoplasmosis after kidney grafting [J].