Quantification of Airborne Influenza and Avian Influenza Virus in a Wet Poultry Market using a Filter/Real-time qPCR Method

被引:35
作者
Chen, Pei-Shih [1 ]
Lin, Chien Kun [1 ]
Tsai, Feng Ta [1 ]
Yang, Chun-Yuh [1 ]
Lee, Chien-Hung [1 ]
Liao, Yen-Shung [1 ]
Yeh, Ching-Ying [2 ]
King, Chwan-Chuen [3 ]
Wu, Jiunn-Lin [4 ]
Wang, Yu-Chun [5 ]
Lin, Kuei-Hsiang [6 ]
机构
[1] Kaohsiung Med Univ, Inst & Dept Publ Hlth, Coll Hlth Sci, Kaohsiung 807, Taiwan
[2] Taipei Med Univ, Sch Med, Taipei, Taiwan
[3] Natl Taiwan Univ, Coll Publ Hlth, Inst Epidemiol, Taipei 10764, Taiwan
[4] Natl Chung Hsing Univ, Coll Sci, Dept Comp Sci, Taichung 40227, Taiwan
[5] Chung Yuan Christian Univ, Coll Engn, Dept Bioenvironm Engn, Chungli, Taiwan
[6] Kaohsiung Med Univ, Dept Clin Lab, Kaohsiung 807, Taiwan
关键词
MYCOBACTERIUM-TUBERCULOSIS; RELATIVE-HUMIDITY; AIR SAMPLES; PCR ASSAY; A H5N1; INACTIVATION; SURVIVAL; TRANSMISSION; IMPINGER; STORAGE;
D O I
10.1080/02786820802621232
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
Influenza viruses cause pandemics in humans. The aim of this study was therefore to evaluate filter/real-time qPCR to quickly and accurately determine and quantify the airborne influenza and avian influenza virus. In this study, the sampling stress of filtration to influenza virus and the storage effects for both the virus and extracted RNA were evaluated in the laboratory. Then, the collection efficiencies of open-face and closed-face filter cassettes were compared in a wet poultry market. From July 8 to August 19, 2006, a total of 36 air samples were quantified by filter/real-time qPCR. The recovery rate of the virus on a filter stored at 4C reached 0.94 after 3-day storage, whereas the RNA stored at -80C was 100% after 3-month storage. In terms of collection efficiency, the closed-face filter cassette was superior to the open-face filter cassette in the field study. In the wet poultry market, it was revealed that both the positive rate and concentration of influenza A virus in the chicken pen were higher than that in the duck pen, possibly due to differences in ventilation type, climate factors, and avian characteristics. To our knowledge, this is the first report describing the quantification of airborne influenza virus in field samples. This quantitative technique should provide more insight into influenza/avian influenza virus transmissibility and epidemiology of influenza/avian influenza, as well as infection control.
引用
收藏
页码:290 / 297
页数:8
相关论文
共 42 条
[1]   Inactivation of viruses in bubbling processes utilized for personal bioaerosol monitoring [J].
Agranovski, IE ;
Safatov, AS ;
Borodulin, AI ;
Pyankov, OV ;
Petrishchenko, VA ;
Sergeev, AN ;
Agafonov, AP ;
Ignatiev, GM ;
Sergeev, AA ;
Agranovski, V .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (12) :6963-6967
[2]  
Aintablian N, 1998, INFECT CONT HOSP EP, V19, P918
[3]   PCR FOR BIOAEROSOL MONITORING - SENSITIVITY AND ENVIRONMENTAL INTERFERENCE [J].
ALVAREZ, AJ ;
BUTTNER, MP ;
STETZENBACH, LD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (10) :3639-3644
[4]  
Areechokchai Darin, 2006, MMWR Suppl, V55, P3
[5]   Concentration profiles of airborne Mycobacterium tuberculosis in a hospital [J].
Chen, Pei-Shih ;
Li, Chih-Shan .
AEROSOL SCIENCE AND TECHNOLOGY, 2008, 42 (03) :194-200
[6]   Quantification of airborne Mycobacterium tuberculosis in health care setting using real-time qPCR coupled to an air-sampling filter method [J].
Chen, PS ;
Li, CS .
AEROSOL SCIENCE AND TECHNOLOGY, 2005, 39 (04) :371-376
[7]   Sampling performance for bioaerosols by flow cytometry with fluorochrome [J].
Chen, PS ;
Li, CS .
AEROSOL SCIENCE AND TECHNOLOGY, 2005, 39 (03) :231-237
[8]  
Dinh PN, 2006, EMERG INFECT DIS, V12, P1841
[9]   Large-scale sequencing of human influenza reveals the dynamic nature of viral genome evolution [J].
Ghedin, E ;
Sengamalay, NA ;
Shumway, M ;
Zaborsky, J ;
Feldblyum, T ;
Subbu, V ;
Spiro, DJ ;
Sitz, J ;
Koo, H ;
Bolotov, P ;
Dernovoy, D ;
Tatusova, T ;
Bao, YM ;
St George, K ;
Taylor, J ;
Lipman, DJ ;
Fraser, CM ;
Taubenberger, JK ;
Salzberg, SL .
NATURE, 2005, 437 (7062) :1162-1166
[10]   AIRBORNE MICRO-ORGANISMS - SURVIVAL TESTS WITH 4 VIRUSES [J].
HARPER, GJ .
JOURNAL OF HYGIENE, 1961, 59 (04) :479-+