A quorum-sensing autoinducer enhances the mexAB-oprM efflux-pump expression without the MexR-mediated regulation in Pseudomonas aeruginosa

被引：34

作者：

Sawada, I

Maseda, H

Nakae, T

Uchiyama, H

Nomura, N ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058572, Japan

[2] Tokai Univ, Sch Med, Dept Mol Life Sci, Isehara, Kanagawa 2591193, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2004年 / 48卷 / 05期

关键词：

quorum-sensing; MexR; MexAB-OprM; MexT;

D O I：

10.1111/j.1348-0421.2004.tb03533.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have previously described that the quorum-sensing autoinducer, N-butyryl-L-homoserine lactone (C4-HSL) enhances mexAB-oprM expression, and this C4-HSL-mediated enhancement of mexAB-oprM expression was repressed by MexT, a positive regulator of the mexEF-oprN operon. In this study, we investigated the interaction between C4-HSL and mexR by using a knockout mutant. It was indicated that the C4-HSL-mediated enhancement of mexAB-oprM expression occurred without MexR-mediated regulation. Furthermore, it was observed that the C4-HSL-mediated enhancement of mexAB-oprM expression without the MexR-mediated regulation was repressed by MexT.

引用

收藏

页码：435 / 439

页数：5

相关论文

共 20 条

[1] The MexR repressor of the mexAB-oprM multidrug efflux operon in Pseudomonas aeruginosa:: Characterization of mutations compromising activity [J].

Adewoye, L ;

Sutherland, A ;

Srikumar, R ;

Poole, K .

JOURNAL OF BACTERIOLOGY, 2002, 184 (15) :4308-4312

[2] MexR repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa:: Identification of MexR binding sites in the mexA-mexR intergenic region [J].

Evans, K ;

Adewoye, L ;

Poole, K .

JOURNAL OF BACTERIOLOGY, 2001, 183 (03) :807-812

[3] NEW NORFLOXACIN RESISTANCE GENE IN PSEUDOMONAS-AERUGINOSA PAO [J].

FUKUDA, H ;

HOSAKA, M ;

HIRAI, K ;

IYOBE, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (09) :1757-1761

[4]

Köhler T, 1999, J BACTERIOL, V181, P6300

[5] Influence of the MexA-MexB-OprM multidrug efflux system on expression of the MexC-MexD-OprJ and MexE-MexF-OprN multidrug efflux systems in Pseudomonas aeruginosa [J].

Li, XZ ;

Barré, N ;

Poole, K .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (06) :885-893

[6] Crystal structure of the MexR repressor of the mexRAB-oprM multidrug efflux operon of Pseudomonas aeruginosa [J].

Lim, D ;

Poole, K ;

Strynadka, NCJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (32) :29253-29259

[7] Assignment of the substrate-selective subunits of the MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa [J].

Maseda, H ;

Yoneyama, H ;

Nakae, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (03) :658-664

[8] Variation of the mexT gene, a regulator of the MexEF-OprN efflux pump expression in wild-type strains of Pseudomonas aeruginosa [J].

Maseda, H ;

Saito, K ;

Nakajima, A ;

Nakae, T .

FEMS MICROBIOLOGY LETTERS, 2000, 192 (01) :107-112

[9] Enhancement of the mexAB-oprM efflux pump expression by a quorum-sensing autoinducer and its cancellation by a regulator, MexT, of the mexEF-oprN efflux pump operon in Pseudomonas aeruginosa [J].

Maseda, H ;

Sawada, I ;

Saito, K ;

Uchiyama, H ;

Nakae, T ;

Nomura, N .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (04) :1320-1328

[10] A novel assembly process of the multicomponent xenobiotic efflux pump in Pseudomonas aeruginosa [J].

Maseda, H ;

Kitao, M ;

Eda, S ;

Yoshihara, E ;

Nakae, T .

MOLECULAR MICROBIOLOGY, 2002, 46 (03) :677-686