Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatis -: art. no. R42

被引：34

作者：

Brunelle, BW

Nicholson, TL

Stephens, RS ^{[1
]}

机构：

[1] Univ Calif Berkeley, Program Infect Dis, Berkeley, CA 94720 USA

[2] Univ Calif San Francisco, Francis I Proctor Fdn, San Francisco, CA 94143 USA

来源：

GENOME BIOLOGY | 2004年 / 5卷 / 06期

关键词：

D O I：

10.1186/gb-2004-5-6-r42

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

By comparing two fully sequenced genomes of Chlamydia trachomatis using competitive hybridization on DNA microarrays, a logarithmic correlation was demonstrated between the signal ratio of the arrays and the 75-99% range of nucleotide identities of the genes. Variable genes within 14 uncharacterized strains of C. trachomatis were identified by array analysis and verified by DNA sequencing. These genes may be crucial for understanding chlamydial virulence and pathogenesis.

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