Automated capillary liquid chromatography for, simultaneous determination of neuroactive amines and amino acids

被引：40

作者：

McKenzie, JAM ^{[1
]}

Watson, CJ ^{[1
]}

Rostand, RD ^{[1
]}

German, I ^{[1
]}

Witowski, SR ^{[1
]}

Kennedy, RT ^{[1
]}

机构：

[1] Univ Florida, Dept Chem, Gainesville, FL 32611 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 962卷 / 1-2期

关键词：

neurotransmitters; amines; amino acids; catecholamines;

D O I：

10.1016/S0021-9673(02)00533-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the separation and quantitative determination of neuroactive amino acids (aspartate, glutamate, citrulline, arginine, glycine, taurine, gamma-aminobutyric acid) and neuroactive amines (noradrenaline, dopamine and serotonin) in a single chromatographic analysis is presented. The method is based on pre-column derivatization with o-phthalaldehyde and tert.-butyl thiol, on-column preconcentration and separation using 50 mum I.D. packed capillary columns, and detection by amperometry. Mass limits of detection are 80-900 amol for all neurotransmitters with RSDs of 0.71 and 4.6% or better for retention time and peak area, respectively. The method was demonstrated by application to the determination of neurotransmitters in microdialysis samples collected from striatum of live rats and tissue samples extracted from butterfly brains. (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：105 / 115

页数：11

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