Efficient Cre-lox linearisation of BACs: Applications to physical mapping and generation of transgenic animals

被引：18

作者：

Mullins, LJ ^{[1
]}

Kotelevtseva, N ^{[1
]}

Boyd, AC ^{[1
]}

Mullins, JJ ^{[1
]}

机构：

[1] WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 12期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1093/nar/25.12.2539

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Due to the size of BAC, PAC and P1 clones, it is often difficult to construct detailed restriction maps, with large number of restriction fragments leading to ambiguity of mapping data. We report the use of Cre recombinase to linearise and asymmetrically introduce label at the unique loxP site of large loxP-containing clones. Subsequent partial digestion allows the direct ordering of restriction fragments. Additionally, BAC DNA linearised using the Cre-lox system has been used successfully to generate transgenic animals.

引用

页码：2539 / 2540

页数：2

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