Two-dimensional gel electrophoresis of Escherichia coli homogenates: The Escherichia coli SWISS-2DPAGE database

被引：62

作者：

Pasquali, C

Frutiger, S

Wilkins, MR

Hughes, GJ

Appel, RD

Bairoch, A

Schaller, D

Sanchez, JC

Hochstrasser, DF

机构：

[1] CMU,DEPT BIOCHEM MED,GENEVA,SWITZERLAND

[2] UNIV HOSP GENEVA,MED COMP CTR,GENEVA,SWITZERLAND

[3] MACQUARIE UNIV,CTR ANALYT BIOTECHNOL,N RYDE,NSW,AUSTRALIA

[4] UNIV GENEVA,DEPT BIOCHEM,CH-1211 GENEVA,SWITZERLAND

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 03期

关键词：

Escherichia coli; SWISS-2DPAGE database; two-dimensional polyacrylamide gel electrophoresis; immobilized pH gradients; protein pattern comparison;

D O I：

10.1002/elps.1150170325

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerous Escherichia coli proteins have already been characterized by two-dimensional gel electrophoresis (2-D PAGE), using carrier ampholytes in the first dimension (VanBogelen, R. A., Sankar, P., Clark, R. L., Began, J. A. and Neidhardt, F. C., Electrophoresis 1992, 13, 1014-1054). We present here a reference protein map of E. coli obtained with immobilized pH gradients (IPG) and available in a SWISS-2DPAGE format. Out of the protein spots identified in the E. coli gene protein database by Neidhardt's group, 153 have been identified on the E.coli SWISS-2DPAGE database map by gel comparison and most of them were confirmed either by the analysis of amino acid composition (AAC) and/or N-terminal microsequencing. Additionally, five as yet unsequenced proteins were found. The E. coli SWISS-2DPAGE database is part of the ExPASy molecular biology server accessible through the Word Wide Web network.

引用

页码：547 / 555

页数：9

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[1] PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HUMAN P50(CSK) PROTEIN-TYROSINE KINASE FROM AN ESCHERICHIA-COLI EXPRESSION SYSTEM OVERPRODUCING THE BACTERIAL CHAPERONES GROES AND GROEL [J].