Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli

被引：580

作者：

Chin, JW

Santoro, SW

Martin, AB

King, DS

Wang, L

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 31期

关键词：

D O I：

10.1021/ja027007w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins. Copyright © 2002 American Chemical Society.

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页码：9026 / 9027

页数：2

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