Fluorescein Redirects a Ruthenium-Octaarginine Conjugate to the Nucleus

被引：234

作者：

Puckett, Cindy A. ^{[1
]}

Barton, Jacqueline K. ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 25期

关键词：

CELL-PENETRATING PEPTIDES; LIGHT-SWITCH; DNA; INTERCALATORS; COMPLEXES; MEMBRANE; DELIVERY; ARGININE; INTERNALIZATION; LOCALIZATION;

D O I：

10.1021/ja9025165

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The cellular uptake and localization of a Ru-octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a fluorophore. Ru-octaarginine-fluorescein stains the cytosol, nuclei, and nucleoli of HeLa cells under conditions where the Ru-octaarginine conjugate without fluorescein shows only punctate cytoplasmic labeling. At higher concentrations, however, Ru-octaarginine without the fluorescein tag does exhibit cytoplasmic, nuclear, and nucleolar staining. Attaching fluorescein to Ru-octaarginine lowers the threshold concentration required for diffuse cytoplasmic labeling and nuclear entry. Hence, the localization of the fluorophore-bound peptide cannot serve as a proxy for that of the free peptide.

引用

页码：8738 / +

页数：3

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