Wallerian degeneration after crush or chronic constriction injury of rodent sciatic nerve is associated with a depletion of endoneurial interleukin-10 protein

被引：47

作者：

George, A ^{[1
]}

Kleinschnitz, C ^{[1
]}

Zelenka, M ^{[1
]}

Brinkhoff, J ^{[1
]}

Stoll, G ^{[1
]}

Sommer, C ^{[1
]}

机构：

[1] Univ Wurzburg, Neurol Klin, D-97080 Wurzburg, Germany

来源：

EXPERIMENTAL NEUROLOGY | 2004年 / 188卷 / 01期

关键词：

peripheral nerve injury; cytokines; epineurium; endoneurium; macrophages; neuropathic pain;

D O I：

10.1016/j.expneurol.2004.02.011

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We used enzyme-linked immunoassay (ELISA), immunohistochemistry (IHC), and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to determine whether interleukin (IL)-10 protein is changed after unilateral crush or chronic constriction injury (CCI) of mouse or rat sciatic nerve and whether IL-10 protein and mRNA are differentially regulated. In the mouse sciatic nerve, IL-10 protein declined rapidly to 10-20% of baseline early after crush or CCI, while the IL10 mRNA was up-regulated with a maximum on Days 1 and 3. In the rat sciatic nerve, IL-10 protein was significantly reduced on Day 3 after CCI, and IL-10 mRNA was up-regulated in both models. These results suggest that changes of the local cytokine network during wallerian degeneration include an early deficiency of the anti inflammatory cytokine IL-10 despite up-regulation at the mRNA level. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：187 / 191

页数：5

共 27 条

[1]

Be'eri M, 1998, EUR J NEUROSCI, V10, P2707

[2] A PERIPHERAL MONONEUROPATHY IN RAT THAT PRODUCES DISORDERS OF PAIN SENSATION LIKE THOSE SEEN IN MAN [J].