Characterisation of autolytic enzymes in Lactobacillus pentosus

被引：12

作者：

Cibik, R

Chapot-Chartier, MP

机构：

[1] INRA, Unite Biochim & Struct Prot, F-78352 Jouy En Josas, France

[2] Univ Uludag, Fac Vet Med, Dept Food Hyg & Technol, Bursa, Turkey

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2004年 / 38卷 / 06期

关键词：

autolysis; Lactobacillus pentosus; nonstarter lactic acid bacteria; peptidoglycan hydrolase; renaturing SDS-PAGE;

D O I：

10.1111/j.1472-765X.2004.01516.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To characterize autolysis and autolytic system of the lactic acid bacterium Lactobacillus pentosus. Methods and Results: Autolysis of nine Lact. pentosus strains was evaluated in buffer solution. Their peptidoglycan hydrolase profiles were examined by renaturing SDS-PAGE and revealed two major activity bands at 58 and 112 kDa. Specificity analysis indicated the presence of at least two different types of peptidoglycan hydrolase activities in Lact. pentosus 1091. Conclusions: Autolysis of Lact. pentosus was shown to be strain dependent and involvement of at least two different autolysins was evidenced. Significance and Impact of the Study: The autolytic system of Lact. pentosus was characterized for the first time and the data obtained could be used in the selection of strains of technological interest.

引用

页码：459 / 463

页数：5

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