Oral tolerance elicited in mice by β-lactoglobulin entrapped in biodegradable microspheres

被引：39

作者：

Pecquet, S

Leo, E

Fritsché, R

Pfeifer, A

Couvreur, P

Fattal, E

机构：

[1] Nestec SA, Nestle Res Ctr, Food Immunol, CH-1000 Lausanne 26, Switzerland

[2] Fac Pharm, URA CNRS 1218, Pharm Galen Lab, F-92296 Chatenay Malabry, France

来源：

VACCINE | 2000年 / 18卷 / 13期

关键词：

oral tolerance; beta-lactoglobulin; microspheres;

D O I：

10.1016/S0264-410X(99)00384-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Oral administration of antigen is known to be appropriate for some vaccine purposes as well as oral tolerance induction. In the present study, oral administration of beta-lactoglobulin (BLC) loaded poly(D,L-lactide-co-glycolide) (D,L-PLG) microspheres induced tolerance was evaluated. A single feeding of 5 mu g of encapsulated BLG tolerised BALB/c mice to subsequent BLG parenteral challenge, suppressing the specific humoral, intestinal and cellular responses. The tolerogenic efficient dose was then reduced 10,000 times, compared to oral administration of soluble BLG. This suggests that loading food proteins into D,L-PLG microspheres might be a potential tool for inducing oral tolerance with allergens. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1196 / 1202

页数：7

共 39 条

[1] Protective immunity against Salmonella typhimurium elicited in mice by oral vaccination with phosphorylcholine encapsulated in poly(DL-lactide-co-glycolide) microspheres [J].